Publication: Estrogen-deficient osteoporosis enhances the recruitment and activity of osteoclasts by breast cancer cells
Authors
Salamanna, Francesca ; Pagani, Stefania ; Maglio, Melania ; Borsari, Veronica ; Giavaresi, Gianluca ; Martelli, Alberto M. ; Buontempo, Francesca ; Fini, Milena
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Publisher
Universidad de Murcia. Departamento de Biología Celular e Histología
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DOI
10.14670/HH-11-651
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info:eu-repo/semantics/article
Description
Abstract
To reduce the burden of bone metastases, the
pathophysiology of the metastatic niche should be
elucidated and targeted. The aim of the present study
was to assess the effect of tumor cells on osteoclast (OC)
recruitment and activity in the presence of altered bone
remodelling. Peripheral blood mononuclear cells
(PBMC) were isolated from healthy and ovariectomized
(OVX) rats and co-cultured with MRMT-1 rat breast
carcinoma cells or with their conditioned medium for 1
and 2 weeks. Alamar Blue viability test, synthesis of
cathepsin K, transforming growth factor-beta 1 (TGFβ1), tumor necrosis factor alpha (TNF-α), vascular
endothelial growth factor (VEGF), metalloproteinase
(MMP)-7, MMP-9, FITC-conjugate phalloidin staining
and tartrate-resistant acid phosphatase (TRAP) staining
were evaluated.
The results indicate that breast carcinoma cells
induced different responses in PBMC derived from rats
affected by estrogen deficiency osteoporosis (OP) in
comparison with healthy ones, with a significant
increase in proliferation rate, OC differentiation,
synthesis of TNF-α, MMP-7 and MMP-9.
The data support the “proof of concept” that OP due
to estrogen deficiency might offer a receptive site for
cancer cells to form bone metastases.
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Citation
Histology and Histopathology, vol.31, nº 1, (2016)
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