Publication: MicroRNAs expression in normal and malignant colon tissues as biomarkers of colorectal cancer and in response to pomegranate extracts consumption: critical issues to discern between modulatory effects and potential artefacts
Loading...
Date
2015-07-20
Authors
Núñez Sánchez, María A. ; Dávalos, Alberto ; González-Sarrías, Antonio ; Casas-Agustench, Patricia ; Visioli, Francesco ; Monedero Saiz, Tamara ; García Talavera, Noelia V. ; Gómez Sánchez, María B. ; Sánchez-Álvarez, Carmen ; García-Albert, Ana M. ; Rodríguez-Gil, Francisco J. ; Ruiz-Marín, Miguel ; Pastor Quirante, Francisco A. ; Martínez Díaz, Francisco ; Tomás-Barberán, Francisco A. ; García-Conesa, María Teresa ; Espín, Juan Carlos
item.page.secondaryauthor
item.page.director
Publisher
Wiley, Wiley-VCH Verlag
publication.page.editor
publication.page.department
DOI
https://doi.org/10.1002/mnfr.201500357
item.page.type
info:eu-repo/semantics/article
Description
© 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. This document is the Published version of a Published Work that appeared in final form in Molecular Nutrition & Food Research. To access the final edited and published work see https://doi.org/10.1002/mnfr.201500357
Abstract
Scope: MicroRNAs (miRs) are proposed as colorectal cancer (CRC) biomarkers. Pomegranate ellagic acid and their microbiota metabolites urolithins exert anticancer effects in preclinical CRC models, and target normal and malignant colon tissues in CRC patients. Herein, we investigated whether the intake of pomegranate extract (PE) modified miRs expression in surgical colon tissues versus biopsies from CRC patients.
Methods and results: We conducted a randomized, double-blind, controlled trial. Thirty-five CRC patients consumed 900 mg PE daily before surgery. Control CRC patients (no PE intake, n = 10) were included. Our results revealed: (1) significant differences for specific miRs between malignant and normal tissues modifiable by the surgical protocols; (2) opposed trends between -5p and -3p isomolecules; (3) general induction of miRs attributable to the surgery; (4) moderate modulation of various miRs following the PE intake, and (5) no association between tissue urolithins and the observed miRs changes. Conclusion: PE consumption appears to affect specific colon tissue miRs but surgery critically alters miRs levels hindering the discrimination of significant changes caused by dietary factors and the establishment of genuine differences between malignant and normal tissues as biomarkers. The components responsible for the PE effects and the clinical relevance of these observations deserve further research.
publication.page.subject
Citation
Mol. Nutr. Food Res. 2015, 59, 1973–1986
item.page.embargo
Collections
Ir a Estadísticas
Sin licencia Creative Commons.