β-lapachone induced cell death in human hepatoma (HepA2) cells

Lai, C.C.; Liu, T. J.; Ho, L. K.; Don, M. J.; Chau, Y. P.

Publication:
β-lapachone induced cell death in human hepatoma (HepA2) cells

dc.contributor.author	Lai, C.C.
dc.contributor.author	Liu, T. J.
dc.contributor.author	Ho, L. K.
dc.contributor.author	Don, M. J.
dc.contributor.author	Chau, Y. P.
dc.date.accessioned	2020-05-22T15:02:47Z
dc.date.available	2020-05-22T15:02:47Z
dc.date.issued	1998
dc.description.abstract	In present study we studied the cytotoxic effects of B-lapachone, a potent anticancer drug, on the human hepatoma cell line (HepA2) under serum-free condition. Most cells died after 2 ,uM B-Iapachone addition at 48 hours. No apoptotic characteristics of DNA ladder was documented by agarose DNA electrophoresis. The blockage of cell cycle at S phase and unscheduled DNA synthesis were demonstrated by flow cytometric analysis and anti-bromodeoxyuridine immunocytochemistry. Ultrastructural observation showed that the swollen mitochondria, dilatation and vesiculation of rER and proliferation of peroxisome-like granules appeared within the cytoplasm of HepA2 cells following drug treatment. Using enzyme cytochemistry, both peroxidase and acid phosphatase activities but not catalase activity were localised in these peroxisome-like granules. Therefore, these results suggested that (a) 13- lapachone has a novel cytotoxic effect on human hepatoma cell; (2) B-lapachone induces the interruption of the cell cycle and unscheduled DNA synthesis in HepA2 cells; and (3) B-lapachone promotes the proliferation of peroxisome-like granules containing peroxidase and acid phosphatase activities without evidence of catalase activity in hepatoma cell line.	es
dc.format	application/pdf	es
dc.format.extent	9	es
dc.identifier.citation	Histology and histopathology, Vol. 13, nº1 (1998)
dc.identifier.issn	1699-5848
dc.identifier.issn	0213-3911
dc.identifier.uri	http://hdl.handle.net/10201/92548
dc.language	eng	es
dc.publisher	F. Hernández y Juan F. Madrid. Universidad de Murcia. Departamento de Biología Celular e Histología	es
dc.rights	info:eu-repo/semantics/openAccess	es
dc.rights	Attribution-NonCommercial-NoDerivatives 4.0 International	*
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/4.0/	*
dc.subject	Hepatoma cells	es
dc.subject	B-lapachone	es
dc.subject	Cell death	es
dc.subject	Electron microsocpy	es
dc.subject.other	CDU::6 - Ciencias aplicadas::61 - Medicina	es
dc.title	β-lapachone induced cell death in human hepatoma (HepA2) cells	es
dc.type	info:eu-repo/semantics/article	es
dspace.entity.type	Publication	es