Publication: β-lapachone induced cell death in human hepatoma (HepA2) cells
Authors
Lai, C.C. ; Liu, T. J. ; Ho, L. K. ; Don, M. J. ; Chau, Y. P.
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Publisher
F. Hernández y Juan F. Madrid. Universidad de Murcia. Departamento de Biología Celular e Histología
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DOI
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info:eu-repo/semantics/article
Description
Abstract
In present study we studied the cytotoxic
effects of B-lapachone, a potent anticancer drug, on the
human hepatoma cell line (HepA2) under serum-free
condition. Most cells died after 2 ,uM B-Iapachone
addition at 48 hours. No apoptotic characteristics of
DNA ladder was documented by agarose DNA electrophoresis. The blockage of cell cycle at S phase and
unscheduled DNA synthesis were demonstrated by flow
cytometric analysis and anti-bromodeoxyuridine
immunocytochemistry. Ultrastructural observation
showed that the swollen mitochondria, dilatation and
vesiculation of rER and proliferation of peroxisome-like
granules appeared within the cytoplasm of HepA2 cells
following drug treatment. Using enzyme cytochemistry,
both peroxidase and acid phosphatase activities but not
catalase activity were localised in these peroxisome-like
granules. Therefore, these results suggested that (a) 13-
lapachone has a novel cytotoxic effect on human
hepatoma cell; (2) B-lapachone induces the interruption
of the cell cycle and unscheduled DNA synthesis in
HepA2 cells; and (3) B-lapachone promotes the
proliferation of peroxisome-like granules containing
peroxidase and acid phosphatase activities without
evidence of catalase activity in hepatoma cell line.
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Citation
Histology and histopathology, Vol. 13, nº1 (1998)
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