Publication:
Absence of lymphatic vessels in non-functioning bleb capsules of glaucoma drainage devices

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Siggel-35-1521-1531-2020.pdf(15.67 MB)
Date
2020
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Authors
Siggel, Robert ; Schroedl, Falk ; Dietlein, Thomas ; Koch, Konrad R. ; Platzl, Christian ; Kaser-Eichberger, Alexandra ; Cursiefen, Claus ; Heindl, Ludwig M.
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Publisher
Universidad de Murcia, Departamento de Biologia Celular e Histiologia
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DOI
https://doi.org/10.14670/HH-18-300
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info:eu-repo/semantics/article
Description
Abstract
Purpose. To evaluate the presence and appearance of blood and lymphatic vessels in nonfunctioning bleb capsules of glaucoma drainage devices (GDD). Materials and methods. Non-functioning (n=14) GDD-bleb capsules of 12 patients were analyzed by immunohistochemistry for blood vessels (CD31, vascular endothelium), lymphatic vessels (lymphatic vessel endothelial hyaluronan receptor-1 [LYVE-1] and podoplanin) and macrophages (CD68). Results. CD31+++ blood vessels and CD68+ macrophages were detected in the outer layer of all specimens. LYVE-1 immunoreactivity was registered in single non-endothelial cells in 8 out of 14 (57%) bleb capsule specimens. Podoplanin-immunoreactivity was detected in all cases, located in cells and profiles of the collagen tissue network of the outer and/or the inner capsule layer. However, a colocalization of LYVE-1 and podoplanin as evidence for lymphatic vessels was not detected. Conclusions. We demonstrate the presence of bloodvessels but absence of lymphatic vessels in nonfunctioning bleb capsules after GDD-implantation. While the absence of lymphatic vessels might indicate a possible reason for drainage device failure, this needs to be confirmed in upcoming studies, including animal experiments.
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Glaucoma drainage devices , Bleb capsule , Lymphatic tissue , Baerveldt , Lyve-1 , Podoplanin
Citation
Histology and Histopathology Vol. 35, nº12 (2020)
URI
http://hdl.handle.net/10201/126728
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Histology and histopathology Vol.35,nº12 (2020)
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