Publication: Ultracytochemical study of trimetaphosphatase activity during acrosomal formation in the mouse testis
Loading...
Date
1995
Authors
Jin, Q.S. ; Kamata, M. ; García del Saz, E. ; Seguchi, H.
item.page.secondaryauthor
item.page.director
Publisher
Murcia : F. Hernández
publication.page.editor
publication.page.department
DOI
item.page.type
info:eu-repo/semantics/article
Description
Abstract
The localization of Trimetaphosphatase
(TMPase) activity during the acrosomal formation in the
mouse testis was enzyme cytochemically investigated by
the cerium-salt method. In addition to the lysosomes of
the Sertoli cells and the spermatogenic cells in the
seminiferous tubules, positive TMPase activity was
detected in the Golgi complex and in the acrosomal
vesicles of the spermatids, as well as in the acrosomes of
both spermatids and spermatozoa. In the Golgi complex
of the spermatids, TMPase activity was observed in the
first one or two lamellae of the trans-face and in the
small vesicles in the vicinity of the Golgi complex.
TMPase positive reaction was also detected in the
acrosomes of the spermatozoa in the lumina of both the
seminiferous tubules and the epididymal duct. The
localization of this enzyme activity was compared with
that of acid phosphatase (ACPase), as detected by the
cerium-based method, using B-glycerophosphate as
substrate: ACPase activity was completely absent from
the Golgi complex, small vesicles, acrosomal vesicle and
acrosome throughout the entire process of acrosomal
formation. TMPase is thought to become one of the
acrosomal components, and may be involved in the
acrosomal reaction during fertilization. .
publication.page.subject
Citation
item.page.embargo
Ir a Estadísticas
Sin licencia Creative Commons.