Histology and histopathology Vol.10, nº 3 (1995)
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- PublicationOpen Accesslmmunomorphologica characteristics of renal cell carcinoma(Murcia : F. Hernández, 1995) Markovic-Lipkovski, J.; Brasanac, D.; Todorovic, V.; Múller, Gerhard Anton; Múller, Gerhard AntonImmunomorphological characteristics of 27 renal cell carcinoma (RCC): 18 clear cell, 6 granular (chromophilic), 2 chromophobe, 1 spindle cell (sarcomatoid) as well as of 1 oncocytoma, were analyzed. The investigation was performed on cryostat sections by immunoperoxidase technique applying a panel of monoclonal antibodies which defined: proximal (TNE3, TN5, 5D9) and distal (TN8, TN9, 7C2) tubular antigens; intercellular adhesion molecule 1 (ICAMl); HLA class I1 (-DQ, -DR and -DP) antigens, intermediary filaments (cytokeratin and vimentin); and antigens on tumour infiltrating mononuclear leucocytes (TT1, TT2 and LeuM3 for CD4, CD8 and CD14 antigens, respectively). All RCC with exception of chromophobe CO-expressed cytokeratin and vimentin. In addition, they were usually positive for all proximal and two distal tubular markers (TN8, TN9) indicating primitive cells which could differentiate into the epithelium of both parts of tubule system as the most probable originators of in RCC. Almost all RCC but the chromophobe aberrantly expressed HLA class I1 antigens which great variability from case to caie. The presence of HLA-DR antigens was more intensive and widespread than of HLA-DQ and-DP antigens. Expression of ICAMl mostly correlated with presence of HLA class I1 antigens, particularly with -DR on tumour cells of RCC HLA-DR antigen expression was always more prominent than mononuclear cell infiltrate (among which macrophages prevailed over T cells) which could suggest that increased histocompatibility antigen expression precedes mononuclear cell influx. In contrast to all other RCC, chromophobe tumours had quite distinct features revealing the most intense reaction with 7C2 (MAb that produced the weakest reaction with other tumour types), absence of vimentin and very weak reaction with antibodies for HLA class Il Ag and ICAM 1. Since oncocytoma has similar immunohistological properties it could be supposed that both tumours have common histogenesis.
- PublicationOpen AccessEffect of chronic alcoholism on neuronal nuclear size and neuronal population in the mammillary body and the anterior thalamic complex of man(Murcia : F. Hernández, 1995) Belzunegui, T.; Insausti, R.; Ibáñez, J.; Gonzalo, L. M.-
- PublicationOpen AccessNuclear vlimata and aneuploidy in embryonic cells is caused by meiosis. Behaviour and properties of meiotic cells(Murcia : F. Hernández, 1995) Logothetou-Rella, H.This study demonstrates that human embryonic cells divide by meiosis. The use of trophoblastic tissue cells (early embryo) and amniotic cells (late embryo) exhibited the following characteristic events of meiosis: nuclear (NVs) and nucleolar (NuVs) vlimata formation; NV invasion in host cells; extrusion of chromosomes; nuclear fusion; metaphase fusion; hybrid cell formation; nuclear, nucleolar and cytoplasmic bridges, chromosomal transfer, variablesized nuclei; nuclear fragmentation; condensed meiotic chromosomes; "0" chromosome; and aneuploidy. Two types of nuclear bridges (NBs) were identified and defined as communicative tubules through which chromosomal transfer among cells is achieved. The wall of NBs is an extension of the nuclear membrane and the lumen contained chromosomal fusion substance (CFS). Embryonic cells formed glycosaminoglycan-sacs (GSGsacs) and rivulets, forming a cytoplasmic communicative system. The extracellular matrix (ECM), GSG-sacs and CFS were composed of glycosaminoglycan-bound protease. The protease which immuno-crossreacted with the al-chymotrypsin antiserum was the meiotic calciumactivated neutral proteinase (CANP). Cytogenetic analysis of early embryonic cells showed higher ratio of aneup1oidy:diploidy than late embryonic cells. The results are discussed in terms of differentiation-mitosis and undifferentiation-meiosis. These observations lead to an embryonic cell life cycle identical to that of malignant cells as follows: meiosis invasion Zygote & NVs and NuVs - recipient host nuclear fusion cells - hybrid cells-w organogenesis metaphase fusion + mitosis
- PublicationOpen AccessModifications in the distribution of met-enkephalin in the limbic system of the cat brain after electroacupuncture. An immunocytochemical study(Murcia : F. Hernández, 1995) Vazquez, J.; Muñoz Madrid, Juan; Cáceres, J.L.The distribution of met-enkephalin in the limbic system of the cat brain and its modification after low frequency electroacupuncture (EA) stimulation have been studied experimentally using the indirect immunocytochemistry technique. A marked increase of post-stimulation met-enkephalin immunoreactivity was observed in the tractus habenulo-penduncularis, tractus mamilo-thalamicus, and media1 forebrain bundle, and a decrease at the leve1 of the nucleus interpeduncularis, medialis dorsalis, stria terminals, septalis lateralis, septalis medialis, accumbens septi, supraopticus, and amygdaloideus centralis. The experimental results link the changes in immunoreactivity (and therefore the structures in which they take place) with the action of low frequency EA, and permit the conclusion that the met-enkephalinergic portion of the limbic system studied is directly related morpho-functionally with analgesia and the anatomic pathways of pain.
- PublicationOpen AccessMacrophages in the external muscle layers of mammalian intestines(Murcia : F. Hernández, 1995) Mikkelsen, H.B.The literature on macrophages in the muscularis extema of mouse, rat, guinea pig, cat, dog and human gut is reviewed. In smaller mammals macrophages are regularly situated in two locations: in the serosa and at the level of Auerbach's plexus between the longitudinal and circular muscle layers. In addition a few solitary cells are present at the level of the deep muscular plexus. At the level of Auerbach's plexus the macrophages occur as a constant and regularly distributed cell population with intimate associations between macrophages and interstitial cells of Cajal. Morphologically they differ from most resident macrophages in being irregular in shape with 4-6 primary cytoplasmic processes, which branch and give a stellate appearance. They have been demonstrated with endocytotic markers (trypan red, FWC-dextran, cholera toxin), immunocytochemically with macrophage antibodies (F4180, M1170) and antibodies against MHC class-I1 antigen, GABA and cGMP. In muscularis externa of the human gut a regularly distributed cell population of macrophages is not obvious. However, a phenotypically distinct subgroup is identified by light microscopy with the pan macrophage antibodies (EBM11, C3bl and partly by ~1 5 0 . 9 5a)n~d shows MHC class-I1 antigen. By electron microscopy muscularis extema macrophages, in all species investigated, appear to be endocytically downregulated, and since they are lysozyme, prostaglandine H synthase (both constitutive and activated) and acid phosphatase negative, they appear to be inactivated cells. Both origin and function of these cells are unknown. They may be immunocompetent, participate in a neuroimmune axis, tissue growth and modulation or other regulations of specific cell functions.