Publication:
Isolation of pluripotent stem cells from human third molar dental pulp

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dc.contributor.authorAtari, M.
dc.contributor.authorBarajas, M.
dc.contributor.authorHernández-Alfaro, F.
dc.contributor.authorGil, C.
dc.contributor.authorFabregat, M.
dc.contributor.authorFerrés Padró, E.
dc.contributor.authorGiner, L.
dc.contributor.authorCasals, N.
dc.date.accessioned2016-05-05T12:21:53Z
dc.date.available2016-05-05T12:21:53Z
dc.date.issued2011
dc.description.abstractPotent stem/progenitor cells have been isolated from normal human dental pulps, termed dental pulp stem cells (DPSCs). However, no study has described the presence of stem cell populations in human dental pulp from the third molar with embryonic phenotypes. The dental pulp tissue was cultured in media with the presence of LIF, EGF, and PDGF. In the present study, we describe a new population of pluripotent stem cells that were isolated from dental pulp (DPPSC). These cells are SSEA-4+, Oct4+, Nanog+, FLK-1+, HNF3beta+, Nestin+, Sox2+, Lin28+, c-Myc+, CD13+, CD105+, CD34- , CD45- , CD90low, CD29+, CD73low, STRO-1low and CD146- . We have investigated by SEM analysis and q-RT-PCR the capacity of DPPSCs to 3D differentiate in vitro using the Cell Carrier 3D glass scaffold into tissues that have similar characteristics to embryonic mesoderm and endoderm layers. These data would support the use of these cells, which are derived from an easily accessible source and can be used in future regeneration protocols for many tissue types that differentiate from the three embryonic layers.es
dc.formatapplication/pdfes
dc.format.extent14es
dc.identifier.issn1699-5848
dc.identifier.issn0213-3911
dc.identifier.urihttp://hdl.handle.net/10201/49561
dc.languageenges
dc.publisherEditores F. Hernandez y Juan F. Madrid. Murcia, Universidad de Murcia, Departamento de Biologia Celular e Histologiaes
dc.relation.ispartofHistology and Histopathology, vol26,nº8, 2011es
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.subjectDental pulpes
dc.subjectPluripotencyes
dc.subjectEmbryonic markerses
dc.subject.other61 - Medicinaes
dc.titleIsolation of pluripotent stem cells from human third molar dental pulpes
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
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Histology and histopathology Vol.26, nº8 (2011)