Publication: Isolation of pluripotent stem cells from human third molar dental pulp
Authors
Atari, M. ; Barajas, M. ; Hernández-Alfaro, F. ; Gil, C. ; Fabregat, M. ; Ferrés Padró, E. ; Giner, L. ; Casals, N.
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Publisher
Editores F. Hernandez y Juan F. Madrid. Murcia, Universidad de Murcia, Departamento de Biologia Celular e Histologia
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Potent stem/progenitor cells have been
isolated from normal human dental pulps, termed dental
pulp stem cells (DPSCs). However, no study has
described the presence of stem cell populations in human
dental pulp from the third molar with embryonic
phenotypes. The dental pulp tissue was cultured in
media with the presence of LIF, EGF, and PDGF. In the
present study, we describe a new population of
pluripotent stem cells that were isolated from dental pulp
(DPPSC). These cells are SSEA-4+, Oct4+, Nanog+,
FLK-1+, HNF3beta+, Nestin+, Sox2+, Lin28+, c-Myc+,
CD13+, CD105+, CD34-
, CD45-
, CD90low, CD29+,
CD73low, STRO-1low and CD146-
. We have investigated
by SEM analysis and q-RT-PCR the capacity of DPPSCs
to 3D differentiate in vitro using the Cell Carrier 3D
glass scaffold into tissues that have similar
characteristics to embryonic mesoderm and endoderm
layers. These data would support the use of these cells,
which are derived from an easily accessible source and
can be used in future regeneration protocols for many
tissue types that differentiate from the three embryonic
layers.
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