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Immunohistochemical evaluation of intermediate filament nestin in dog hair follicles

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dc.contributor.authorMercati, F.es
dc.contributor.authorPascucci, L.es
dc.contributor.authorGargiulo, A.M.
dc.contributor.authorDall’Aglio, C.
dc.contributor.authorCeccarelli, P.
dc.date.accessioned2013-01-22T10:03:10Z
dc.date.available2013-01-22T10:03:10Z
dc.date.issued2008
dc.description.abstractHair follicles (HFs) are self-renewing structures that reconstitute themselves through the hair cycle. They maintain reservoirs of stem cells (SC) that are thought to reside in the bulge area, a region localized in the lowermost permanent portion of HFs. In mice and humans, HF bulge cells express nestin and present stem features as pluripotency. Nestin is a class VI intermediate filament protein; it was first described as a specific marker of CNS stem cells, but recent studies suggest that it may represent a more general stem cell marker (Wiese et al., 2004; Hoffman, 2006). Bulge cell characteristics have mainly been studied in mice and humans, but recently, a bulge-like region was identified also in dog HFs (Pascucci et al., 2006). In this work we investigate the presence and localization of nestin in dog HFs with the aim of evaluating its expression and to correlate it with the location of the bulge-like region. Immunostaining of skin samples collected from healthy dogs was performed by using a rabbit anti-nestin polyclonal antibody. The presence of a population of immunoreactive cells was revealed in the hair follicle middle region, at the arrector pili muscle insertion level. An immunohistochemical signal was detected only in primary hair follicles throughout the hair cycle. These observations led us to conclude that nestin positive cells are located in the bulge-like region of dog HFs and strengthen our hypothesis regarding the correlation between this region and the dog HF stem compartment.es
dc.formatapplication/pdfes
dc.format.extent7es
dc.identifier.issn0213-3911es
dc.identifier.urihttp://hdl.handle.net/10201/29828
dc.languageenges
dc.publisherMurcia : F. Hernándezes
dc.relation.ispartofHistology and histopathologyes
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.subjectStem celles
dc.subjectHair folliclees
dc.subjectDog
dc.subject.other636 - Veterinaria. Explotación y cría de animales. Cría del ganado y de animales domésticoses
dc.titleImmunohistochemical evaluation of intermediate filament nestin in dog hair follicleses
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
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Histology and histopathology Vol.23, nº9 (2008)