Publication: Immunohistochemical evaluation of intermediate filament nestin in dog hair follicles
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Date
2008
Authors
Mercati, F. ; Pascucci, L. ; Gargiulo, A.M. ; Dall’Aglio, C. ; Ceccarelli, P.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Hair follicles (HFs) are self-renewing
structures that reconstitute themselves through the hair
cycle. They maintain reservoirs of stem cells (SC) that
are thought to reside in the bulge area, a region localized
in the lowermost permanent portion of HFs. In mice and
humans, HF bulge cells express nestin and present stem
features as pluripotency. Nestin is a class VI
intermediate filament protein; it was first described as a
specific marker of CNS stem cells, but recent studies
suggest that it may represent a more general stem cell
marker (Wiese et al., 2004; Hoffman, 2006).
Bulge cell characteristics have mainly been studied
in mice and humans, but recently, a bulge-like region
was identified also in dog HFs (Pascucci et al., 2006). In
this work we investigate the presence and localization of
nestin in dog HFs with the aim of evaluating its
expression and to correlate it with the location of the
bulge-like region.
Immunostaining of skin samples collected from
healthy dogs was performed by using a rabbit anti-nestin
polyclonal antibody. The presence of a population of
immunoreactive cells was revealed in the hair follicle
middle region, at the arrector pili muscle insertion level.
An immunohistochemical signal was detected only in
primary hair follicles throughout the hair cycle.
These observations led us to conclude that nestin
positive cells are located in the bulge-like region of dog
HFs and strengthen our hypothesis regarding the
correlation between this region and the dog HF stem
compartment.
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