Publication:
Effects of irradiation on intestinal cells in vivo and in vitro

Simple item page
dc.contributor.authorWróblewski, R.es
dc.contributor.authorWróblewski, J.es
dc.contributor.authorJalnäs, M.
dc.contributor.authorVan Decker, G.
dc.contributor.authorBjörk, J.
dc.contributor.authorRoomans, G.M.
dc.date.accessioned2011-05-11T10:57:23Z
dc.date.available2011-05-11T10:57:23Z
dc.date.issued2002
dc.description.abstractThe effects of irradiation on intestinal epithelial cells were analyzed in vivo and in vitro. The in vivo study was carried out on the rat small intestine and for the in vitro study the intestinal crypt cell-line IEC-6 was used. Rat intestine and IEC-6 cells were irradiated with X-ray doses ranging between 1-16 Gy. Energ y - dispersive X-ray microanalysis was used for detection of the elemental changes in the cells. Cell morphology was investigated in the scanning electron microscope, DNAsynthesis by autoradiography of 3H - t h y m i d i n e incorporating nuclei and proliferation by cell counting. Our results indicate that in vivo, in the crypt cells, the increasing doses of irradiation led to increased sodium and lowered potassium and phosphorus concentrations. Corresponding ion shifts were found in the irradiated IEC-6 cells. Cells continued to proliferate up to the dose of 8 Gy, although the proliferation rate became lower with increasing dose of irradiation. The increasing dose of irradiation significantly reduced DNA-synthesis (16 Gy decreased DNA-synthesis by 50%) which resulted in a complete inhibition of cell proliferation. Analysis of goblet cells also showed characteristic radiationdependent elemental changes. Scanning electron microscopical investigation of cells in culture revealed that most of the control cells were flat and had rather smooth cell membranes. Irradiation led to the appearance of numerous different membrane manifestations (microvilli of varying length and distribution, and blebs). Frequency of differences in the topology of the cells was related to the dose of irradiation. Our study clearly demonstrates that even low doses of irradiation cause changes in the ionic composition of the cells and inhibit DNA-synthesis and cell proliferation. The effects observed in the crypt cells in vivo were the same as in the intestinal cell line i n v i t ro, which indicates that IEC-6 cells can be used for investigation of side effects of radiation to the abdomen.es
dc.formatapplication/pdfes
dc.format.extent13es
dc.identifier.issn0213-3911es
dc.identifier.urihttp://hdl.handle.net/10201/20736
dc.languageenges
dc.publisherMurcia : F. Hernándezes
dc.relation.ispartofHistology and histopathologyes
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.subjectIntestinees
dc.subjectIrradiationes
dc.subject.otherCDU::6 - Ciencias aplicadas::61 - Medicinaes
dc.titleEffects of irradiation on intestinal cells in vivo and in vitroes
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Effects of irradiation on intestinal cells in vivo and in vitro.pdf
Size:
3.13 MB
Format:
Adobe Portable Document Format
Description:
Download
Collections
Histology and histopathology Vol.17, nº 1 (2002)