Publication: Proteoglycans of alveolar bone of diabetic and non-diabetic mice, a histochemical study
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Date
1987
Authors
Johnson, R. B.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
The effect of diabetes mellitus on the
interdental alveolar bone has been long debated. The
present study reported the distribution of
glycosaminoglycans (GAG) in normal and diabetic
alveolar bone using histochemical techniques. Animals
were rendered diabetic and killed at 2,4,6 and 9 weeks
after injections. Tissues were stained with Alcian blue
8GX dye (pH 2.5) to demonstrate GAG and the intensity
of the staining reactions compared with age-matched
controls. During the experiment, weights of control
animals did not change significantly; weights of diabetic
animals were significantly less than initial weights from O-
6 weeks (p<0.001), but became nearly equal by 9 weeks.
Staining intensity of diabetic bone demonstrated initial
decrease (0-4 weeks) followed by a marked increase (4-9
weeks) suggesting an early decline in bone GAG levels
followed by increased bone GAG levels as compared to
age-matched control and initial levels. Bone GAG levels
were significantly different between diabetics and agematched
controls at 2 (p<0.005) 4 (p<0.001), 6
(p<0.001) and 9 (p<0.001) weeks after streptozotocin
injections. Digestion with chondroitinase AC, ABC and
streptomyces hyaluronidase suggested significant differences between control and diabetic bone matrix in
the levels of chondroitin 4 and 6 sulfates (p<0.05) and
hyaluronic acid (p<0.001) but not dermatan sulfate. In
control and diabetic bone, chondroitin sulfates were
located within the bone matrix, dermatan sulfate within
bone matrix and Sharpey fiber bundles. Thus, bone
GAG changes in diabetes mellitus occur predominately
within bone matrix and are variable depending on the
length of the disease.
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