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Effects of N-acetylcysteine on human ovarian tissue preservation undergoing cryopreservation procedure

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dc.contributor.authorFabbri, Raffaella
dc.contributor.authorSapone, Andrea
dc.contributor.authorPaolini, Moreno
dc.contributor.authorVivarelli, Fabio
dc.contributor.authorFranchi, Paola
dc.contributor.authorLucarini, Marco
dc.contributor.authorPasquinelli, Gianandrea
dc.contributor.authorVicenti, Rossella
dc.contributor.authorMacciocca, Maria
dc.contributor.authorVenturoli, Stefano
dc.contributor.authorCanistro, Donatella
dc.date.accessioned2020-09-18T11:26:57Z
dc.date.available2020-09-18T11:26:57Z
dc.date.issued2015
dc.description.abstractThe aim of the study was to evaluate the effects of the antioxidant N-acetylcysteine (NAC), added in freezing/thawing solutions, on reactive oxygen species (RRS) levels and on ovarian tissue preservation after cryopreservation. Ovarian samples from 10 subjects suffering from cancer diseases were cryopreserved using the slow freezing/rapid thawing standard protocol without or with NAC supplementation. RRS levels produced during cryopreservation were monitored by electron paramagnetic resonance (EPR) spectroscopy. The preservation of fresh ovarian tissue (t0), thawed tissue (t1 and t1 NAC) and thawed tissue maintained at 4°C for 2 hrs (t2 and t2 NAC) was analysed by light microscopy, transmission electron microscopy, Ki67 immunohistochemical and TUNEL analysis. It was possible to design a maximum peak for RRS production at t1, which slightly decreased at t2. NAC reduced the extent of RRS levels in cryopreserved ovarian tissues if compared with non-supplemented ones, although not restoring RRS production to baseline values. Comparative analysis between the two cryopreservation protocols showed that a better preservation of morphological characteristics, proliferation index and DNA integrity of ovarian tissue was obtained using NAC and no differences between t1NAC and t2NAC were observed. The employment of NAC during cryopreservation procedure could be anuseful strategy for preserving the function of endogenous cellular systems. Nevertheless, further studies on the viability of thawed ovarian tissue are needed to support the feasibility of this approach in clinical settings.es
dc.formatapplication/pdfes
dc.format.extent11es
dc.identifier.citationHistology and Histopathology, vol. 30, nº 6, (2015)
dc.identifier.doi10.14670/HH-30.725
dc.identifier.issn1699-5848
dc.identifier.urihttp://hdl.handle.net/10201/96142
dc.languageenges
dc.publisherF. Hernández y Juan F. Madrid. Universidad de Murcia: Departamento de Biología Celular e Histologíaes
dc.relationSin financiación externa a la universidades
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectCryopreservation of human ovarian tissuees
dc.subjectReactive radical specieses
dc.subjectN-acetylcysteinees
dc.subjectElectron paramagnetic resonance spectroscopyes
dc.subjectLight microscopyes
dc.subjectTransmission electron microscopyes
dc.subjectKi67 immunohistochemicales
dc.subjectTerminal deoxynucleotidyl transferase dUTP nick end labelling (TUNEL).es
dc.subject.otherCDU::6 - Ciencias aplicadas::61 - Medicina::616 - Patología. Medicina clínica. Oncologíaes
dc.titleEffects of N-acetylcysteine on human ovarian tissue preservation undergoing cryopreservation procedurees
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
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Histology and histopathology Vol.30, nº6 (2015)