Publication: Expression of claudin-1, -3, -4, -5 and -7 proteins in low grade colorectal carcinoma of canines
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Date
2010
Authors
Jakab, Cs. ; Rusvai, M. ; Gálfi, P. ; Szabó, Z. ; Szabára, Á. ; Kulka, J.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
The aim of the present study was to
characterise the expression pattern of claudin-1, -3, -4,
-5 and -7 tight junction proteins in canine normal
colorectum and in the low-grade, tubulopapillary
colorectal carcinoma in canines. Methods and results:
The biopsy samples included 10 canine normal
colorectal tissues and 20 canine low grade colorectal
carcinomas (CLGCCs). The canine normal colorectal
mucosa was negative for claudin-1. Claudin-1 was
detected as a non-diffuse intense membrane labelling of
neoplastic epithelial cells in low grade colorectal cancer
in canines. Fifty five per cent of all tumours showed a
weak cytoplasmic pattern of staining for claudin-1
protein. The normal colorectal mucosa showed diffuse
punctate positivity for claudin-3. Claudin-3 was detected
as an intense lateral membrane labelling of tumour cells
in CLGCCs. Claudin-4 expression in surface and crypt
epithelial cells of the intact colorectal mucosa in canines
was punctate. Claudin-4 molecule was detected as a
lateral membrane labelling of neoplastic cells in
CLGCCs. The epithelium of the CLGCCs and the low
grade colorectal carcinoma were negative for claudin-5.
The surface and crypt epithlial cells of the canine normal
colorectal mucosa showed a diffuse lateral membranous
pattern of staining for claudin-7. Claudin-7 molecule
was detected as an intense membrane labelling of
neoplastic cells in CLGCCs. Seventy per cent of all
tumours showed weak cytoplasmic positivity for
claudin-7. Conclusion: Consequently, we hypothesize
that claudin-1 plays a role in the progression of
CLGCCs. Further functional studies are needed to
clarify the biological role of the mislocalization of the
claudin-1 molecule from cell membrane to the
cytoplasm in CLGCCs. Lower claudin-4 expression
suggests that reduced expression of claudin-4 molecule
may lead to cellular disorientation, detachment and
invasion of CLGCCs. Further functional studies are
needed to clarify the biological role of overexpression
and mislocalisation of claudin-7 in CLGCCs.
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