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The use of slaughterhouse-obtained small intestinal tissue as control material in histological studies should be applied with prudence

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dc.contributor.authorDe Ceulaer, K.
dc.contributor.authorVan Ginneken, C.
dc.contributor.authorDelesalle, C.
dc.contributor.authorVan Brantegem, L.
dc.contributor.authorDeprez, P.
dc.contributor.authorWeyns, A.
dc.date.accessioned2016-04-04T15:02:16Z
dc.date.available2016-04-04T15:02:16Z
dc.date.issued2011
dc.description.abstractThis study aimed to evaluate the reliability of slaughterhouse-obtained small intestinal tissue as control material in equine colic research where molecular stress responses in small intestinal tissue are investigated. For this purpose, small intestinal samples from colic horses were collected during surgery or immediately after euthanasia at the oral border of strangulation resection sites and routinely processed for histopathology (i.c. rinsed with 4°C Krebs’ solution, fixated overnight with 4% neutral buffered formaldehyde (FH) at room temperature). Control samples consisted of pieces of mid-jejunum, collected at the slaughterhouse and routinely processed for histopathology under 4 different conditions. The 4 conditions differed with regard to incubation and fixation temperature and whether or not oxygenated Krebs’ solution was used. Histological scoring revealed that slaughterhouse samples had a higher mean lesion score (P<0.001) than colic samples. In addition, more slaughterhouse samples had a higher mean inflammation score than colic samples (P=0.001). The inflammatory cells in the small intestine consisted mostly of eosinophils and as such were very suggestive for parasitic infestation. Hypoxia-inducible factor-1α (HIF1α) nuclear immunoreactivity was more pronounced in slaughterhouse tissue, probably as a result of the delay between slaughter and sampling (P=0.034). The histopathological score (P=0.291), the inflammation score (P=0.248) and the HIF1α nuclear immunoreactivity (P=0.538) did not differ between the different collection protocols. It is concluded that slaughterhouseobtained small intestinal tissue shows distinct alterations and that its use as control tissue when evaluating molecular stress responses should be applied with prudence.es
dc.formatapplication/pdfes
dc.format.extent5es
dc.identifier.issn1699-5848
dc.identifier.issn0213-3911
dc.identifier.urihttp://hdl.handle.net/10201/48459
dc.languageenges
dc.publisherMurcia: F. Hernándezes
dc.relation.ispartofHistology and histopathology, Vol. 26, nº 4 (2011)es
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.subjectHorse intestinees
dc.subjectHistologyes
dc.subject.other636 - Veterinaria. Explotación y cría de animales. Cría del ganado y de animales domésticoses
dc.titleThe use of slaughterhouse-obtained small intestinal tissue as control material in histological studies should be applied with prudencees
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
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Histology and histopathology Vol.26, nº4 (2011)