Publication: The use of slaughterhouse-obtained
small intestinal tissue as control material in
histological studies should be applied with prudence
Authors
De Ceulaer, K. ; Van Ginneken, C. ; Delesalle, C. ; Van Brantegem, L. ; Deprez, P. ; Weyns, A.
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Publisher
Murcia: F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
This study aimed to evaluate the reliability of
slaughterhouse-obtained small intestinal tissue as control
material in equine colic research where molecular stress
responses in small intestinal tissue are investigated. For
this purpose, small intestinal samples from colic horses
were collected during surgery or immediately after
euthanasia at the oral border of strangulation resection
sites and routinely processed for histopathology (i.c.
rinsed with 4°C Krebs’ solution, fixated overnight with
4% neutral buffered formaldehyde (FH) at room
temperature). Control samples consisted of pieces of
mid-jejunum, collected at the slaughterhouse and
routinely processed for histopathology under 4 different
conditions. The 4 conditions differed with regard to
incubation and fixation temperature and whether or not
oxygenated Krebs’ solution was used. Histological
scoring revealed that slaughterhouse samples had a
higher mean lesion score (P<0.001) than colic samples.
In addition, more slaughterhouse samples had a higher
mean inflammation score than colic samples (P=0.001).
The inflammatory cells in the small intestine consisted
mostly of eosinophils and as such were very suggestive
for parasitic infestation. Hypoxia-inducible factor-1α
(HIF1α) nuclear immunoreactivity was more
pronounced in slaughterhouse tissue, probably as a result
of the delay between slaughter and sampling (P=0.034).
The histopathological score (P=0.291), the inflammation
score (P=0.248) and the HIF1α nuclear immunoreactivity
(P=0.538) did not differ between the different
collection protocols. It is concluded that slaughterhouseobtained
small intestinal tissue shows distinct alterations
and that its use as control tissue when evaluating molecular stress responses should be applied with
prudence.
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