Publication: The glomerular distribution of laminin and fibronectin in glomerulonephritis
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Date
1993
Authors
Nakopoulou, Lydia ; Stefanak, K. ; Zeis, P.M. ; Papadakis, J. ; Boletis, J. ; Vosnidis, G. ; Davaris, P.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Laminin (LAM) and fibronectin (FI) are
regarded as major components of the glomerular
extracellular matrix
The aim of this study was to define the distribution of
LAM and F1 in primary glomerulonephritis (GN) and
GN of systemic lupus erythematosus (SLE) and to
correlate the type of glomerular disorders with possible
changes in the expression of these components. Normal
portions of kidney tissue from 10 patients with renal
tumors and sixty-six renal biopsies obtained from
patients with GN were studied by the immunoperoxidase-
antiperoxidase (PAP) method for the
detection of LAM and FI. Twelve patients had
membranous GN (MGN), 8 mesangiocapillary GN
(MCGN), 2 1 mesangioproliferative GN (MPGN),
including I l cases of IgA nephropathy, 11 focal
segmental glomerulosclerosis (FSGS) and 14 had SLE.
In MGN, LAM was detected more intensely than F1
along the glomerular basement membranes (GBM), in
subepithelial GBM protrusions and in the newly-formed
GBM. On the contrary, F1 was intensely expressed in the
mesangium. LAM and FI expression was pronounced in
stages I1 and 111 of MGN. In MCGN, LAM and F1 were
diffusely expressed along the GBM and in the
mesangium. The distribution of the two antigens in
MPGN and FSGS was similar to that seen in normal
glomeruli. However, the F1 staining reaction was more
intense in severe mesangioproliferative lesions, mainly
observed in the cases of IgA-nephropathy, There were
no differences in the distribution of LAM and F1
between primary and SLE GN. The antigen staining
pattern was pronounced in the membranous and
mesangiocapillary lesions of SLE GN. The crescents
observed in 7 cases contained increased amounts of both
LAM and FI, while the adhesions with Bowman's
capsule seen in 9 cases demonstrated increased amounts
of LAM. In contrast, the large adhesions observed in 2
cases and the sclerotic lesions in 4 cases contained only
small amounts of LAM.
Offprint requests to: Dr. Lydia Nakopoulou. Department of Pathology.
Medical School, University of Athens, 138-140 Grigoriou Afxediou,
Alhens 15772, Greece
In conclusion, the increased LAM and F1 glomerular
expression mainly in MGN and MCGN, and the F1
overexpression in severe mesangioproliferative lesions
of IgA nephropathy suggest that the disturbance of
extracellular glomerular matrix is probably due to the
damage of glomerular cells or the involvement of the
above components in immune-complex formation.
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