Publication:
Identification of changes in serum analytes and possible metabolic pathways associated with canine obesity-related metabolic dysfunction

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Date
2019-02
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Authors
Tvarijonaviciute, Asta ; Barić Rafaj, R. ; Horvatic, A. ; Muñoz Prieto, A. ; Guillermin, N. ; Tumpa, A. ; Cerón, J. J. ; Martínez Subiela, S. ; Mrljak, V.
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Publisher
Elsevier
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Medicina y Cirugía Animal
DOI
https://doi.org/10.1016/j.tvjl.2018.12.006
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info:eu-repo/semantics/article
Description
© 2018 Elsevier Ltd. This document is the Published version of a Published Work that appeared in final form in The Veterinary Journal. To access the final edited and published work see https://doi.org/10.1016/j.tvjl.2018.12.006
Abstract
The main objective of this study was to identify analytes that could change and that could help to clarify the metabolic and physiopathological changes related to canine obesity-related metabolic dysfunction (ORMD). For this, serum from 35 overweight/obese dogs, with and without ORMD, was submitted to a comprehensive panel of biochemistry analysis, a gel-free tandem mass tag isobaric label-based proteomic analysis, and, finally, selected proteins were used as a starting point for creating a protein interaction network. Dogs with ORMD showed significantly higher serum concentrations of alanine aminotransferase (ALT), alkaline phosphatase (ALP), Ca, total proteins, albumin, total cholesterol, triglycerides, glucose, and butyrylcholinesterase (BChE) activity in comparison with dogs without ORMD. Proteomic analysis revealed that 23 proteins related to lipid metabolism, the complement factor system, cellular adhesion and functionality, inflammation, and coagulation were altered in dogs with ORMD. Finally, the obtained protein interaction network highlighted that the central term of this network was the negative regulation of the immune response. These data suggest that canine ORMD is associated with changes in analytes that reflect altered lipid metabolism, and liver and immune function impairment and suggests the potential for a prothrombotic state and lung function alterations.
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Dog , Metabolic syndrome , Obesity , Protein network , Proteomics
Citation
The Veterinary Journal , 2019, Vol. 244, pp. 51-59
URI
http://hdl.handle.net/10201/148502
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