Salivary D-dimer in pigs: Validation of an automated assay and changes after acute stress

López Martínez, M. J.; Escribano, D.; Contreras Aguilar, M. D.; García Martínez, Juan Diego; Martínez Subiela, S.; Cerón, J. J.

Publication:
Salivary D-dimer in pigs: Validation of an automated assay and changes after acute stress

dc.contributor.author	López Martínez, M. J.
dc.contributor.author	Escribano, D.
dc.contributor.author	Contreras Aguilar, M. D.
dc.contributor.author	García Martínez, Juan Diego
dc.contributor.author	Martínez Subiela, S.
dc.contributor.author	Cerón, J. J.
dc.contributor.department	Medicina y Cirugía Animal
dc.date.accessioned	2025-01-30T11:19:57Z
dc.date.available	2025-01-30T11:19:57Z
dc.date.issued	2020-05-31
dc.description	© 2020 Elsevier Ltd. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ This document is the Published version of a Published Work that appeared in final form in The Veterinary Journal . To access the final edited and published work see https://doi.org/10.1016/j.tvjl.2020.105472	es
dc.description.abstract	D-dimer is a peptide found in serum and is derived from the degradation of blood clots. Even though it has been analysed in human saliva, D-dimer has not been previously evaluated in the saliva of any veterinary species, and its source and role remain unknown. The objectives of this research were firstly, to validate the use of an automated method for the measurement of D-dimer in porcine saliva, and secondly, to evaluate whether D-dimer concentration changes in pig saliva after an acute stress stimulus. For this purpose, a complete analytical validation of a commercially-available immunoturbidimetric assay was carried out. In addition, an experimental acute stress model was induced in 11 pigs based on a technique involving restraint by nose-snare immobilisation for 1 min. Saliva samples were subsequently collected at different times and D-dimer, salivary alpha-amylase (sAA) and cortisol were assessed in order to evaluate changes in its concentrations after the stress induction. The D-dimer automated assay showed adequate reproducibility and sensitivity, with coefficients of variation below 10% and a limit of quantification of 0.167 μg/mL fibrinogen equivalent units (FEU). It also showed a high accuracy, determined by linearity under dilution and recovery tests. In the stress model, a significant increase (P < 0.05) in salivary D-dimer 15 min after the stress stimulus and a positive correlation between D-dimer and sAA (r = 0.51; P < 0.001) were observed. These results indicate that D-dimer can be measured in porcine saliva with an automated method and suggest that its concentration can be influenced by stressful conditions.	es
dc.format	application/pdf	es
dc.format.extent	25	es
dc.identifier.citation	The Veterinary Journal, 2020, Vol. 259–260 : 105472
dc.identifier.doi	https://doi.org/10.1016/j.tvjl.2020.105472
dc.identifier.issn	Print: 1090-0233
dc.identifier.issn	Electronic: 1532-2971
dc.identifier.uri	http://hdl.handle.net/10201/149733
dc.language	eng	es
dc.publisher	Elsevier	es
dc.relation	This study was supported by grant 19894/GERM/15 of the Seneca Foundation of Region of Murcia (Groups of Excellence). María J. López-Martínez was funded by a pre-doctoral training contract for research staff financed by the CARM Ministry of Employment, Research and Universities, through the Seneca Foundation - Science and Technology Agency of the Region of Murcia. Damián Escribano was funded on a post-doctoral contract (IJC2018-035105-I) “Juan de la Cierva Incorporación” supported by the “Ministerio de Economía y Competitividad”, Spain. María D. Contreras-Aguilar was funded on a predoctoral contract “FPU” from the University of Murcia (Spain), grant number R-605/2016. Part of this work was submitted as a graduate project by María J. López Martínez.	es
dc.relation.publisherversion	https://www.sciencedirect.com/science/article/pii/S1090023320300496?via%3Dihub	es
dc.rights.accessRights	info:eu-repo/semantics/restrictedAccess
dc.subject	Alpha amylase	es
dc.subject	Cortisol	es
dc.subject	D dimer	es
dc.subject	Saliva	es
dc.subject	Stress	es
dc.subject	Swine	es
dc.title	Salivary D-dimer in pigs: Validation of an automated assay and changes after acute stress	es
dc.type	info:eu-repo/semantics/article	es
dspace.entity.type	Publication	es