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LncRNA ADAMTS9-AS1 knockdown restricts cell proliferation and EMT in non-small cell lung cancer

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dc.contributor.authorLi, Zhongwen
dc.contributor.authorYue, Guojun
dc.contributor.authorZhang, Tingyou
dc.contributor.authorWu, Jinzhi
dc.contributor.authorTian, Xin
dc.date.accessioned2023-01-26T11:39:51Z
dc.date.available2023-01-26T11:39:51Z
dc.date.issued2021
dc.description.abstractA recent bioinformatics analysis identified long nonā€coding RNA antisense 1 ADAMTS9-AS1 as an independent prognostic marker in several tumors, including prostate cancer and bladder cancer. Nevertheless, the prognostic value and functional role of ADAMTS9-AS1 in non-small cell lung cancer (NSCLC) remain elusive. Here, we first found that the expression of ADAMTS9-AS1 was significantly upregulated in NSCLC tissues compared with adjacent normal tissues using quantitative real time PCR analysis. Clinically, we observed that ADAMTS9-AS1 expression was associated with TNM stage, lymph node metastasis and poor prognosis in NSCLC patients. By performing lossof-function assay in A549 and 95D cells, our in vitro experiments further showed that knockdown of ADAMTS9-AS1 remarkedly suppressed cell proliferation, caused cell cycle G0/G1 arrest and apoptosis, and inhibited cell migration and invasion in NSCLC cells using CCK-8, colony formation, flow cytometry and transwell assays. Moreover, we found that ADAMTS9-AS1 knockdown downregulated the expression of CDK4, N-cadherin, Vimentin, but upregulated the expression of Bad and E-cadherin. In summary, our results revealed that ADAMTS9-AS1 may serve as a potential therapeutic target for the treatment of patients with NSCLCes
dc.formatapplication/pdfes
dc.format.extent10es
dc.identifier.citationHistology and Histopathology Vol. 36, nĀŗ10 (2021)
dc.identifier.doihttps://doi.org/10.14670/HH-18-347
dc.identifier.issn0213-3911
dc.identifier.issn1699-5848
dc.identifier.urihttp://hdl.handle.net/10201/127899
dc.languageenges
dc.publisherUniversidad de Murcia, Departamento de Biologia Celular e Histiologiaes
dc.relationSin financiaciĆ³n externa a la Universidades
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectADAMTS9-AS1es
dc.subjectNSCLCes
dc.subjectPrognosises
dc.subjectProliferationes
dc.subjectEMTes
dc.subject.otherCDU::6 - Ciencias aplicadas::61 - Medicina::616 - PatologĆ­a. Medicina clĆ­nica. OncologĆ­aes
dc.titleLncRNA ADAMTS9-AS1 knockdown restricts cell proliferation and EMT in non-small cell lung canceres
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
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Histology and histopathology Vol.36,nĀŗ10 (2021)