Publication: LncRNA ADAMTS9-AS1 knockdown restricts cell proliferation and EMT in non-small cell lung cancer
Authors
Li, Zhongwen ; Yue, Guojun ; Zhang, Tingyou ; Wu, Jinzhi ; Tian, Xin
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Publisher
Universidad de Murcia, Departamento de Biologia Celular e Histiologia
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DOI
https://doi.org/10.14670/HH-18-347
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info:eu-repo/semantics/article
Description
Abstract
A recent bioinformatics analysis identified
long nonācoding RNA antisense 1 ADAMTS9-AS1 as an
independent prognostic marker in several tumors,
including prostate cancer and bladder cancer.
Nevertheless, the prognostic value and functional role of
ADAMTS9-AS1 in non-small cell lung cancer (NSCLC)
remain elusive. Here, we first found that the expression
of ADAMTS9-AS1 was significantly upregulated in
NSCLC tissues compared with adjacent normal tissues
using quantitative real time PCR analysis. Clinically, we
observed that ADAMTS9-AS1 expression was
associated with TNM stage, lymph node metastasis and
poor prognosis in NSCLC patients. By performing lossof-function assay in A549 and 95D cells, our in vitro
experiments further showed that knockdown of
ADAMTS9-AS1 remarkedly suppressed cell
proliferation, caused cell cycle G0/G1 arrest and
apoptosis, and inhibited cell migration and invasion in
NSCLC cells using CCK-8, colony formation, flow
cytometry and transwell assays. Moreover, we found that
ADAMTS9-AS1 knockdown downregulated the
expression of CDK4, N-cadherin, Vimentin, but
upregulated the expression of Bad and E-cadherin. In
summary, our results revealed that ADAMTS9-AS1 may
serve as a potential therapeutic target for the treatment of
patients with NSCLC
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Citation
Histology and Histopathology Vol. 36, nĀŗ10 (2021)
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Este Ćtem estĆ” sujeto a una licencia Creative Commons. http://creativecommons.org/licenses/by-nc-nd/4.0/