Validation of three commercially available immunoassays for quantification of IgA, IgG, and IgM in porcine saliva samples

Escribano Tortosa, Damián; Gutiérrez Montes, Ana María; Martínez Subiela, Silvia; Tecles Vicente, Fernando; Cerón Madrigal, José Joaquín

Publication:
Validation of three commercially available immunoassays for quantification of IgA, IgG, and IgM in porcine saliva samples

dc.contributor.author	Escribano Tortosa, Damián
dc.contributor.author	Gutiérrez Montes, Ana María
dc.contributor.author	Martínez Subiela, Silvia
dc.contributor.author	Tecles Vicente, Fernando
dc.contributor.author	Cerón Madrigal, José Joaquín
dc.contributor.department	Producción Animal
dc.date.accessioned	2025-01-15T13:07:34Z
dc.date.available	2025-01-15T13:07:34Z
dc.date.issued	2011-10-22
dc.description	© 2011 Elsevier Ltd. All rights reserved. This document is the Published version of a Published Work that appeared in final form in Research in Veterinary Science. To access the final edited and published work see https://doi.org/10.1016/j.rvsc.2011.09.018	es
dc.description.abstract	The objectives of this study were to perform the optimization and validation of three commercially available immunoassays for the measurement of IgA, IgG, and IgM (Igs) in porcine saliva samples and to determinate if their concentrations may be used to distinguish healthy from diseased animals. Intra and inter assay coefficients of variation were lower than 15% in all cases. All methods showed good linearity and recovery; and detection limits were low enough to detect Igs levels in healthy and diseased animals. The clinical validation showed an increase statistically significant (P < 0.05) in the group of diseased animals versus healthy pigs. Therefore, these assays may be used in porcine saliva samples, in addition, the measurement of Igs in saliva could be a practical tool, simple and minimally invasive, to evaluate the humoral immune status of pigs.	es
dc.format	application/pdf	es
dc.format.extent	6	es
dc.identifier.citation	Research in Veterinary Science 93 (2012) 682–687
dc.identifier.doi	https://doi.org/10.1016/j.rvsc.2011.09.018
dc.identifier.issn	Print: 0034-5288
dc.identifier.issn	Electronic: 1532-2661
dc.identifier.uri	http://hdl.handle.net/10201/148505
dc.language	eng	es
dc.publisher	Elsevier	es
dc.relation	This study was supported by a grant from the Spanish Ministry of Education and Science (AGL 2009-08509).	es
dc.relation.publisherversion	https://www.sciencedirect.com/science/article/pii/S0034528811003778?via%3Dihub
dc.rights.accessRights	info:eu-repo/semantics/restrictedAccess
dc.subject	IgA, IgG, and IgM
dc.subject	Immunoglobulin
dc.subject	Porcine
dc.subject	Saliva
dc.subject	Validation
dc.subject.other	CDU::6 - Ciencias aplicadas::63 - Agricultura. Silvicultura. Zootecnia. Caza. Pesca::636 - Veterinaria. Explotación y cría de animales. Cría del ganado y de animales domésticos	es
dc.title	Validation of three commercially available immunoassays for quantification of IgA, IgG, and IgM in porcine saliva samples	es
dc.type	info:eu-repo/semantics/article	es
dspace.entity.type	Publication	es