Publication: Validation of three commercially available immunoassays for quantification of IgA, IgG, and IgM in porcine saliva samples
Authors
Escribano Tortosa, Damián ; Gutiérrez Montes, Ana María ; Martínez Subiela, Silvia ; Tecles Vicente, Fernando ; Cerón Madrigal, José Joaquín
item.page.secondaryauthor
item.page.director
Publisher
Elsevier
publication.page.editor
publication.page.department
DOI
https://doi.org/10.1016/j.rvsc.2011.09.018
item.page.type
info:eu-repo/semantics/article
Description
© 2011 Elsevier Ltd. All rights reserved. This document is the Published version of a Published Work that appeared in final form in Research in Veterinary Science. To access the final edited and published work see https://doi.org/10.1016/j.rvsc.2011.09.018
Abstract
The objectives of this study were to perform the optimization and validation of three commercially available immunoassays for the measurement of IgA, IgG, and IgM (Igs) in porcine saliva samples and to determinate if their concentrations may be used to distinguish healthy from diseased animals. Intra and inter assay coefficients of variation were lower than 15% in all cases. All methods showed good linearity and recovery; and detection limits were low enough to detect Igs levels in healthy and diseased animals. The clinical validation showed an increase statistically significant (P < 0.05) in the group of diseased animals versus healthy pigs. Therefore, these assays may be used in porcine saliva samples, in addition, the measurement of Igs in saliva could be a practical tool, simple and minimally invasive, to evaluate the humoral immune status of pigs.
publication.page.subject
Citation
Research in Veterinary Science 93 (2012) 682–687
item.page.embargo
Collections
Ir a Estadísticas
Sin licencia Creative Commons.