Publication: How is plasminogen/plasmin system contributing to regulate sperm entry into the oocyte?
Authors
Grullón LA ; Gadea Mateos, Joaquín ; Mondéjar Corbalán, Irene ; Romar Andrés, Raquel ; Matas Parra, Carmen ; Coy Fuster, Pilar
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Facultades de la UMU
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Coy Fuster, Pilar
Publisher
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DOI
10.1177/1933719112473657
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info:eu-repo/semantics/article
Description
Abstract
Plasminogen is present in the oviduct, on the zona pellucida (ZP) and on oolemma, and reduces the number of sperm penetrating the oocyte during in vitro fertilization in pig and cow. It is unknown how this reduction occurs. We tested whether plasminogen (1) changed the ZP resistance to enzymatic digestion thus making the passage of the spermatozoa across it difficult; (2) reduced the sperm functionality, assessed by sperm viability, motility, spontaneous acrosome reaction and membrane lipid disorder; or (3) affected the sperm-ZP binding before or after sperm-ZP interaction. The mechanism by which plasminogen/plasmin system contributes to regulate sperm entry into the oocyte is not inducing a ZP hardening or a decrease in sperm functionality but detaching more than 50% of sperm bound to the ZP. It is suggested that the fertilizing spermatozoon activates plasminogen into plasmin at the oocyte surface and that plasmin removes additional spermatozoa attached to the ZP.
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Citation
Grullón LA, Gadea J, Mondéjar I, Matás C, Romar R, Coy P. How is plasminogen/plasmin system contributing to regulate sperm entry into the oocyte? Reprod Sci. 2013 Sep;20(9):1075-82. doi: 10.1177/1933719112473657. Epub 2013 Feb 18. PMID: 23420828; PMCID: PMC3745708.
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