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Effects of Helicobacter pylori on biological characteristics of gastric epithelial cells

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dc.contributor.authorWang, Ping
dc.contributor.authorMei, Juan
dc.contributor.authorTao, Jing
dc.contributor.authorZhang, Ning
dc.contributor.authorTian, Hua
dc.contributor.authorFu, Guo-Hui
dc.date.accessioned2017-09-08T15:50:05Z
dc.date.available2017-09-08T15:50:05Z
dc.date.issued2012
dc.description.abstractInfection with Helicobacter pylori (H. pylori) strains is linked to an increased risk of inflammation and gastric cancer. To investigate the effects of H. pylori on biological characteristics of gastric epithelial cells SGC-7901, derived from human adenocarcinoma, morphological appearances of both the pathogen and these cells, as well as features of attachment and internalization were observed by using transmission electron microscopy (TEM). We also investigated cell junctions and invasion by TEM and Transwell Invasion Assay. Cell proliferation and apoptosis were assessed by using chromogenic methylthiazol tetrazolium bromide (MTT) dye and flow cytometry. Three types of H. pylori were observed around, attaching to, or invading tumor cells. Cellular damage was characterized by vacuolar degeneration, dilated endoplasmic reticulum (ER), and reduction of organelles. Cell junctions and cell microvilli reduced or disappeared. H. pylori inhibited cell proliferation, whereas it had no effect on apoptosis. It also promoted gastric carcinoma cell invasion. H. pylori damages cell construction, destroys cell junctions, inhibits cell proliferation, promotes cell invasive ability, and, therefore, might accelerate the malignant progress and metastasis of gastric canceres
dc.formatapplication/pdfes
dc.format.extent13es
dc.identifier.citationHistology and histopathology, Vol. 27, nº 8 (2012)
dc.identifier.issn1699-5848
dc.identifier.issn0213-3911
dc.identifier.urihttp://hdl.handle.net/10201/54113
dc.languageenges
dc.publisherF. Hernández y Juan F. Madrid. Universidad de Murcia. Departamento de Biología Celular e Histologíaes
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.subjectGastric carcinomases
dc.subjectHelicobacter pylories
dc.subject.otherCDU::6 - Ciencias aplicadas::61 - Medicina::616 - Patología. Medicina clínica. Oncologíaes
dc.titleEffects of Helicobacter pylori on biological characteristics of gastric epithelial cellses
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
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Histology and histopathology Vol.27, nº 8 (2012)