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Haptoglobin expression in human colorectal cancer

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dc.contributor.authorMariño Crespo, Óscar
dc.contributor.authorCuevas Álvarez, Elisa
dc.contributor.authorHarding, Amy L.
dc.contributor.authorMurdoch, Craig
dc.contributor.authorFernández Briera, Almudena
dc.contributor.authorGil Martín, Emilio
dc.date.accessioned2022-07-07T08:24:22Z
dc.date.available2022-07-07T08:24:22Z
dc.date.issued2019
dc.description.abstractAims and experimental design. The acutephase protein haptoglobin (Hp) has been recently detected in colorectal cancer (CRC) tissue, where its expression correlates with metastasis. Recently, we identified Hp as a CDw75 antigen-expressing protein in colorectal tissue. To deepen the knowledge of this protein in CRC, we studied the expression of Hp in healthy and tumour tissue specimens from 62 CRC patients by immunohistochemistry and Western blotting, as well as in the Caco-2 and HT-29 CRC cell lines by quantitative PCR, immunofluorescence microscopy and flow cytometry. Results and discussion. Hp immunopositive staining was absent in the 18 healthy colorectal specimens analysed, whereas it was observed in 24% (15/62) of the tumour specimens as cytoplasmic granules within cancer cells. Furthermore, Hp expression in CRC was associated with Dukes’ stage and the presence of metastasis in our population of study. In vitro cultured Caco-2 and HT-29 cells expressed mRNA for Hp and the protein was detected at the cell surface. Conclusions. This study confirms the expression of Hp in CRC, both in vivo and in vitro, and provides further evidence of its association with disease progression and metastasis.es
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dc.format.extent11es
dc.identifier.citationHistology and Histopathology, Vol.34, nº8, (2019)
dc.identifier.doiDOI: 10.14670/HH-18-100
dc.identifier.issn1699-5848
dc.identifier.issn0213-3911
dc.identifier.urihttp://hdl.handle.net/10201/122187
dc.languageenges
dc.publisherUniversidad de Murcia. Departamento de Biología Celular e Histologíaes
dc.relationSin financiación externa a la Universidades
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectHaptoglobinaes
dc.subjectHuman colorectal cancer tissuees
dc.subjectMetastasises
dc.subjectCaco-2es
dc.subjectHT-29es
dc.subject.otherCDU::6 - Ciencias aplicadas::61 - Medicina::616 - Patología. Medicina clínica. Oncologíaes
dc.titleHaptoglobin expression in human colorectal canceres
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
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Histology and histopathology Vol.34, nº8 (2019)