Publication: Haptoglobin expression in human colorectal cancer
Authors
Mariño Crespo, Óscar ; Cuevas Álvarez, Elisa ; Harding, Amy L. ; Murdoch, Craig ; Fernández Briera, Almudena ; Gil Martín, Emilio
item.page.secondaryauthor
item.page.director
Publisher
Universidad de Murcia. Departamento de Biología Celular e Histología
publication.page.editor
publication.page.department
DOI
DOI: 10.14670/HH-18-100
item.page.type
info:eu-repo/semantics/article
Description
Abstract
Aims and experimental design. The acutephase protein haptoglobin (Hp) has been recently
detected in colorectal cancer (CRC) tissue, where its
expression correlates with metastasis. Recently, we
identified Hp as a CDw75 antigen-expressing protein in
colorectal tissue. To deepen the knowledge of this
protein in CRC, we studied the expression of Hp in
healthy and tumour tissue specimens from 62 CRC
patients by immunohistochemistry and Western blotting,
as well as in the Caco-2 and HT-29 CRC cell lines by
quantitative PCR, immunofluorescence microscopy and
flow cytometry. Results and discussion. Hp immunopositive staining was absent in the 18 healthy colorectal
specimens analysed, whereas it was observed in 24%
(15/62) of the tumour specimens as cytoplasmic granules
within cancer cells. Furthermore, Hp expression in CRC
was associated with Dukes’ stage and the presence of
metastasis in our population of study. In vitro cultured
Caco-2 and HT-29 cells expressed mRNA for Hp and the
protein was detected at the cell surface. Conclusions.
This study confirms the expression of Hp in CRC, both
in vivo and in vitro, and provides further evidence of its
association with disease progression and metastasis.
publication.page.subject
Citation
Histology and Histopathology, Vol.34, nº8, (2019)
item.page.embargo
Ir a Estadísticas
Este ítem está sujeto a una licencia Creative Commons. http://creativecommons.org/licenses/by-nc-nd/4.0/