Publication: Utilidad de las proteínas de fase aguda en el líquido cefalorraquídeo canino para el diagnóstico de la me-ningoencefalomielitis de origen desconocido
Authors
Verdú Serrano, María Elena ; Muñoz Prieto, Alberto ; Mateo Pampliega, Isidro ; García Martínez, Juan Diego
item.page.secondaryauthor
item.page.director
Publisher
Universidad de Murcia. Servicio de publicaciones
publication.page.editor
publication.page.department
DOI
https://doi.org/10.6018/analesvet.562071
item.page.type
info:eu-repo/semantics/article
Description
Abstract
El sistema inmunitario facilita la defensa de los seres vivos, que se desencadena a través de la respuesta de fase aguda, generándose las proteínas de fase aguda. Estas proteínas, medidas en suero, resultan de utilidad. El uso de biomarcadores para el diagnóstico precoz de enfermedades inflamatorias del sistema nervioso central (SNC), está en auge en medicina humana. En el caso de las meningoencefalomielitis de origen desconocido (MOD), no hay estudios con una información relevante que sea capaz de solventar el gran inconveniente de no poder obtener un diagnóstico definitivo, que no sea de manera postmortem. La medición de proteínas de fase aguda, como la proteína C reactiva (CRP) y la haptoglobina en el líquido cefalorraquídeo (LCR) podría ayudar a solventar este problema. El objetivo de esta investigación es valorar si estas proteínas se pueden detectar en el LCR, mediante un analizador bioquímico automatizado y estudiar la existencia de alteraciones DOI: 10.6018/analesvet.562071 en animales con MOD. Para ello, los animales incluidos en el estudio se dividieron en un grupo control, com-puesto por perros sin MOD y un grupo problema, subdividido en perros con MOD y perros con MOD tratados con corticoides. Para la medición de estos parámetros se utilizó el analizador Olympus AU600®. Para realizar el estudio comparativo entre grupos se aplicó la prueba no paramétrica de Kruskal-Wallis, seguido del test de Dunn para comparaciones múltiples. La CRP no mostró variaciones estadísticamente significativas, mientras que la haptoglobina no se pudo detectar en el LCR, por lo que se debería valorar su medición con técnicas más sensibles que permitan su cuantificación.
The immune system facilitates the defense of living beings, which is triggered through the acute phase response, generating acute phase proteins. These proteins, measured in serum, are useful. The use of biomark-ers for the early diagnosis of inflammatory diseases of the central nervous system (CNS) is booming in human medicine. In the case of meningoencephalomyelitis of unknown origin (MOD), there are no studies with rel-evant information capable of solving the major drawback of not being able to obtain a definitive diagnosis, ex-cept postmortem. The measurement of acute phase proteins, such as C-reactive protein (CRP) and haptoglobin in cerebrospinal fluid (CSF) could help to solve this problem. The aim of this research is to assess whether these proteins can be detected in CSF using an automated biochemical analyzer and to study the existence of altera-tions in MOD animals. For this purpose, the animals included in the study were divided into a control group, composed of dogs without MOD, and a problem group, subdivided into dogs with MOD and dogs with MOD treated with corticoids. The Olympus AU600® analyzer was used to measure these parameters. To perform the comparative study between groups, the non-parametric Kruskal-Wallis test was applied, followed by Dunn’s test for multiple comparisons. CRP did not show statistically significant variations, while haptoglobin could not be detected in CSF, so its measurement should be evaluated with more sensitive techniques that allow its quantification
The immune system facilitates the defense of living beings, which is triggered through the acute phase response, generating acute phase proteins. These proteins, measured in serum, are useful. The use of biomark-ers for the early diagnosis of inflammatory diseases of the central nervous system (CNS) is booming in human medicine. In the case of meningoencephalomyelitis of unknown origin (MOD), there are no studies with rel-evant information capable of solving the major drawback of not being able to obtain a definitive diagnosis, ex-cept postmortem. The measurement of acute phase proteins, such as C-reactive protein (CRP) and haptoglobin in cerebrospinal fluid (CSF) could help to solve this problem. The aim of this research is to assess whether these proteins can be detected in CSF using an automated biochemical analyzer and to study the existence of altera-tions in MOD animals. For this purpose, the animals included in the study were divided into a control group, composed of dogs without MOD, and a problem group, subdivided into dogs with MOD and dogs with MOD treated with corticoids. The Olympus AU600® analyzer was used to measure these parameters. To perform the comparative study between groups, the non-parametric Kruskal-Wallis test was applied, followed by Dunn’s test for multiple comparisons. CRP did not show statistically significant variations, while haptoglobin could not be detected in CSF, so its measurement should be evaluated with more sensitive techniques that allow its quantification
publication.page.subject
Citation
Anales de Veterinaria, vol.38, (2024)
item.page.embargo
Ir a Estadísticas
Este ítem está sujeto a una licencia Creative Commons. http://creativecommons.org/licenses/by-nc-nd/4.0/