A dopamine-based biosynthetic pathway produces decarboxylated betalains in Chenopodium quinoa

Henarejos Escudero, Paula; Contreras-Llano, Luis Eduardo; Lozada-Ramírez, José Daniel; Gomez-Pando, Luz Rayda; Gandía Herrero, Fernando; García Carmona, Francisco

Publication:
A dopamine-based biosynthetic pathway produces decarboxylated betalains in Chenopodium quinoa

dc.contributor.author	Henarejos Escudero, Paula
dc.contributor.author	Contreras-Llano, Luis Eduardo
dc.contributor.author	Lozada-Ramírez, José Daniel
dc.contributor.author	Gomez-Pando, Luz Rayda
dc.contributor.author	Gandía Herrero, Fernando
dc.contributor.author	García Carmona, Francisco
dc.contributor.department	Bioquímica y Biología Molecular "A"
dc.date.accessioned	2025-05-28T07:19:48Z
dc.date.available	2025-05-28T07:19:48Z
dc.date.issued	2021-04-07
dc.description	© 2021, American Society of Plant Biologists. All rights reserved. This document is the Published version of a Published Work that appeared in final form in Plant Physiology. To access the final edited and published work see https://doi.org/10.1093/plphys/kiab158	es
dc.description.abstract	Betalains are the nitrogenous pigments that replace anthocyanins in the plant order Caryophyllales. Here, we describe unconventional decarboxylated betalains in quinoa (Chenopodium quinoa) grains. Decarboxylated betalains are derived from a previously unconsidered activity of the 4,5-DOPA-extradiol-dioxygenase enzyme (DODA), which has been identified as the key enzymatic step in the established biosynthetic pathway of betalains. Here, dopamine is fully characterized as an alternative substrate of the DODA enzyme able to yield an intermediate and structural unit of plant pigments: 6-decarboxy-betalamic acid, which is proposed and described. To characterize this activity, quinoa grains of different colors were analyzed in depth by chromatography, time-of-flight mass spectrometry, and reactions were performed in enzymatic assays and bioreactors. The enzymatic-chemical scheme proposed leads to an uncharacterized family of 6-decarboxylated betalains produced by a hitherto unknown enzymatic activity. All intermediate compounds as well as the final products of the dopamine-based biosynthetic pathway of pigments have been unambiguously determined and the reactions have been characterized from the enzymatic and functional perspectives. Results evidence a palette of molecules in quinoa grains of physiological relevance and which explain minor betalains described in plants of the Caryophyllales order. An entire family of betalains is anticipated.	es
dc.format	application/pdf	es
dc.format.extent	14	es
dc.identifier.citation	Plant Physiology 2021: 186 1473–1486
dc.identifier.doi	https://doi.org/10.1093/plphys/kiab158
dc.identifier.issn	Print: 0032-0889
dc.identifier.issn	Electronic: 1532-2548
dc.identifier.uri	http://hdl.handle.net/10201/155157
dc.language	eng	es
dc.publisher	Oxford University Press, American Society of Plant Biologists
dc.relation	This work was supported by the Ministerio de Ciencia e Innovación, Project AGL2017-86526-P (MCI/AEI/FEDER, UE) and by the Programa de Ayudas a Grupos de Excelencia de la Región de Murcia, Fundación Séneca, Agencia de Ciencia y Tecnología de la Región de Murcia (Project 19893/GERM/ 15). P.H.-E. holds a contract financed by University of Murcia, Spain. L.E.C.-L. was supported through a fellowship from Consejo Nacional de Ciencia y Tecnología (CONACYT, Mexico), and currently holds a UC MEXUS Doctoral Fellowship.	es
dc.relation.publisherversion	https://academic.oup.com/plphys/article/186/3/1473/6214491
dc.rights.accessRights	info:eu-repo/semantics/restrictedAccess
dc.subject.other	CDU::5 - Ciencias puras y naturales::57 - Biología::577 - Bioquímica. Biología molecular. Biofísica	es
dc.title	A dopamine-based biosynthetic pathway produces decarboxylated betalains in Chenopodium quinoa	es
dc.type	info:eu-repo/semantics/article	es
dspace.entity.type	Publication	es
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