Publication: Morphometric study of the umbilical cord in in-vitro-derived pigs
Authors
Álvarez Martín, Úrsula ; Coy, Pilar ; Romar Andrés, Raquel ; Párraga Ros, Ester ; Seva Alcaraz, Juan
item.page.secondaryauthor
item.page.director
Publisher
Springer
publication.page.editor
publication.page.department
DOI
item.page.type
info:eu-repo/semantics/lecture
Description
© 2022, The Author(s) under exclusive licence to University of Navarra. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ This document is the Accepted Manuscript, version of a Published Work that appeared in final form in Journal of Physiology and Biochemistry. To access the final edited and published work see https://doi.org/10.1007/s13105-022-00923-3
Abstract
The umbilical cord is the vital fetus-placenta connection and represents one of the greatest sources of precursor cells. Histologically it is a single amniotic epithelium that encloses a mucoid connective tissue, and a vein and two arteries lacking tunica adventitia. Instead, there is a special mucoid connective tissue named Wharton’s Jelly (WJ) that is divided into a perivascular and an intermediate zone, the first being the most abundant in mesenchymal stromal cells. Morphological charac-teristics of the umbilical cord and its components have been related to fetal malformations, preterm birth and low birth weight. The objective of this study was to compare the WJ and the vascular area in the umbili-cal cord of pigs born from in-vitro- and in-vivo-produced embryos (the latter born by artificial insemination of sows; AI group). In-vitro embryos (IVP) were produced after insemination in-vitro of matured oocytes and further in-vitro culture up to blastocyst stage in media sup-plemented with (RF-IVP group) or without (C-IVP group) reproductive fluids (1% porcine oviductal fluid and 1% uterine fluid). Blastocysts produced were surgically transferred at day 7 post-in-vitro fertiliza-tion. After birth, umbilical cord samples of 15 animals (5 per group) were collected and productive parameters recorded. Samples were fixed (10% buffered formaldehyde solution) and paraffin-embedded. Complete sections of 5 μm thickness were stained (hematoxylin-eosin) and digitized with a Histech MIDI II 3D scanner at 0.172 pixels/μm. The virtual microscope SlideViewer 2.5 3D Histech was used for the digital analysis of the total umbilical area, the thickness of both WJ’s zones, and each vessel’s area. Data were analyzed by one-way ANOVA (SPSS Statistics 28) and the differences were compared by Tukey’s test (P<0.05). The thickness of the WJ-perivascular zone was significantly higher in the C-IVP group (AI: 572,4 ± 39,3 μm; C-IVP: 662,2 ± 42,3 μm; RF-IVP: 501,1 ± 35,7 μm), and was significantly correlated with piglet birth weight, placental weight and placental efficiency (Pear-son <0.05). No significant differences in the WJ-intermediate zone thickness, vascular and total umbilical areas were found. These results might be related to the subsequent development of pathophysiological changes in IVP animals during their growth.
publication.page.subject
Citation
Journal of Physiology and Biochemistry, 2022, Vol. 78, S37
item.page.embargo
Collections
Ir a Estadísticas
Este ítem está sujeto a una licencia Creative Commons. http://creativecommons.org/licenses/by-nc-nd/4.0/