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Evaluation of changes in ion release and biological properties of NeoMTA-Plus and Endocem-MTA exposed to an acidic environment

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dc.contributor.authorRodríguez Lozano, Francisco Javier
dc.contributor.authorOñate Sánchez, Ricardo E.
dc.contributor.authorLopez-García, S.
dc.contributor.authorMoraleda, J. M.
dc.contributor.authorLozano, A.
dc.contributor.authorForner, L.
dc.contributor.authorMurcia, L.
dc.contributor.authorCollado-González, Mar
dc.contributor.authorGarcía Bernal, David
dc.contributor.departmentDermatología, Estomatología, Radiología y Medicina Física
dc.date.accessioned2024-02-06T07:47:27Z
dc.date.available2024-02-06T07:47:27Z
dc.description© 2019 International Endodontic Journal. Published by John Wiley & Sons Ltd. This document is the Published version of a Published Work that appeared in final form in International Endodontic Journal. To access the final edited and published work see https://doi.org/10.1111/iej.13107
dc.description.abstractAim To analyse in vitro changes in ion release and biological properties of Endocem-MTA (Maruchi, Wonju, Korea) and NeoMTA-Plus (Avalon Biomed Inc, Bradenton, FL, USA) exposed to acidic or neutral environment on human dental periodontal ligament stem cells (hPDLSCs). Methodology Cell viability and wound healing assays were performed using eluates of each material. Cell death and changes in phenotype induced by the set endodontic sealer eluates were evaluated through flow cytometry. To evaluate cell attachment to the different materials, hPDLSCs were directly seeded onto the material surfaces and analysed by scanning electron microscopy. The chemical composition of the materials was determined by energy-dispersive X-ray (EDX), and ion release was evaluated by inductively coupled plasma-mass spectrometry. Statistical analysis was performed with analysis of variance and a Bonferroni or Tukey post-test (a < 0.05). Results The MTT assay revealed non-cytotoxic effects of NeoMTA-Plus and Endocem-MTA at pH 5.2 and 7.4. However, there were minor differences compared with the control, especially at pH 5.2, where both materials were associated with significantly greater cell viability (P < 0.05). In both environments, the materials stimulated hPDLSCs to migrate. hPDLSCs were attached to the bioactive cements, with multiple prolongations proliferated on the surface of the samples. Moreover, there were no changes to cell phenotype or apoptosis/necrosis rates, indicating that the acidic environment did not induce cell death. Prismatic crystalline structures were seen on the surface of the cements exposed to butyric acid and EDX analysis identified a marked peak of Ca2+ from NeoMTA-Plus and Endocem-MTA in acidic and physiological environments. Conclusions An acidic environment favoured the release of Ca2+ ions from both bioactive cements, and the cytotoxicity of these bioactive cements was low in both environments studied.es
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dc.identifier.citationInternational Endodontic Journal, 52, 1196–1209, 2019
dc.identifier.issnPrint:0143-2885
dc.identifier.issnElectronic: 1365-2591
dc.identifier.urihttp://hdl.handle.net/10201/138701
dc.languageenges
dc.relationThis work was supported by the Spanish Net of Cell Ther-apy (TerCel), RETICS subprograms of the I + D + I 2013–2016Spanish National Plan, projects “RD12/0019/0001” and“RD16/0011/0001” funded by Instituto de Salud Carlos III toJMM, and co-founded by European Regional DevelopmentFund.es
dc.rights.accessRightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectAcidic environmenten
dc.subjectBioactive endodontic cementsen
dc.subjectBiological propertiesen
dc.subjectIon releaseen
dc.subjectNeoMTA-Plusen
dc.subject.otherMedicinaes
dc.titleEvaluation of changes in ion release and biological properties of NeoMTA-Plus and Endocem-MTA exposed to an acidic environmentes
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
relation.isAuthorOfPublicationba32f760-d55c-40a7-9452-62dfd47cbeaf
relation.isAuthorOfPublication2372b160-a380-45dd-835a-6f377fbeaf97
relation.isAuthorOfPublication.latestForDiscoveryba32f760-d55c-40a7-9452-62dfd47cbeaf
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