Publication: Evaluation of changes in ion release and biological properties of NeoMTA-Plus and Endocem-MTA
exposed to an acidic environment
Authors
Rodríguez Lozano, Francisco Javier ; Oñate Sánchez, Ricardo E. ; Lopez-García, S. ; Moraleda, J. M. ; Lozano, A. ; Forner, L. ; Murcia, L. ; Collado-González, Mar ; García Bernal, David
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Publisher
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DOI
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info:eu-repo/semantics/article
Description
© 2019 International Endodontic Journal. Published by John Wiley & Sons Ltd. This document is the Published version of a Published Work that appeared in final form in International Endodontic Journal. To access the final edited and published work see https://doi.org/10.1111/iej.13107
Abstract
Aim To analyse in vitro changes in ion release and biological properties of Endocem-MTA (Maruchi, Wonju, Korea) and NeoMTA-Plus (Avalon Biomed Inc, Bradenton, FL, USA) exposed to acidic or neutral environment on human dental periodontal ligament stem cells (hPDLSCs).
Methodology Cell viability and wound healing assays were performed using eluates of each material. Cell death
and changes in phenotype induced by the set endodontic sealer eluates were evaluated through flow cytometry. To evaluate cell attachment to the different materials, hPDLSCs were directly seeded onto the material surfaces
and analysed by scanning electron microscopy. The chemical composition of the materials was determined by
energy-dispersive X-ray (EDX), and ion release was evaluated by inductively coupled plasma-mass spectrometry.
Statistical analysis was performed with analysis of variance and a Bonferroni or Tukey post-test (a < 0.05).
Results The MTT assay revealed non-cytotoxic effects of NeoMTA-Plus and Endocem-MTA at pH 5.2 and 7.4. However, there were minor differences compared with the control, especially at pH 5.2, where both materials were associated with significantly greater cell viability (P < 0.05). In both environments, the materials stimulated hPDLSCs to migrate. hPDLSCs were attached to the bioactive cements, with multiple prolongations proliferated on the surface of the samples. Moreover, there were no changes to cell phenotype or apoptosis/necrosis rates, indicating that the acidic environment did not induce cell death. Prismatic crystalline structures were seen on the surface of the cements exposed to butyric acid and EDX analysis identified a marked peak of Ca2+ from NeoMTA-Plus and Endocem-MTA in acidic and physiological environments.
Conclusions An acidic environment favoured the release of Ca2+ ions from both bioactive cements, and
the cytotoxicity of these bioactive cements was low in both environments studied.
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Citation
International Endodontic Journal, 52, 1196–1209, 2019
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