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Toll like receptor-4 expression in lipopolysaccharide induced lung inflammation

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dc.contributor.authorJanardhan, K.es
dc.contributor.authorMcIsaac, M.es
dc.contributor.authorFowlie, J.
dc.contributor.authorShrivastav, A.
dc.contributor.authorCaldwell, S.
dc.contributor.authorSharma, R.K.
dc.contributor.authorSingh, B.
dc.date.accessioned2011-06-30T12:03:11Z
dc.date.available2011-06-30T12:03:11Z
dc.date.issued2006
dc.description.abstractBacterial lipopolysaccharides (LPS) initiate immune response through Toll-like receptor 4 (TLR4). Because many a times host is confronted with secondary bacterial challenges, it is critical to understand TLR4 expression following initial provocation. We studied TLR4 expression in rats at various times after intratracheal instillation of LPS. Although TLR4 mRNA was undetectable in normal lungs, it increased at 6h and 12h and declined at 36h post-LPS treatment. Western blots showed TLR4 protein at all time points. Immunohistochemistry localized TLR4 in alveolar septal cells, bronchial epithelium, macrophages and endothelium of large and peribronchial blood vessels. Dual label immunoelectron microscopy showed colocalization of TLR4 and LPS in the cytoplasm and nucleus of various lung and inflammatory cells. Nuclear localization of TLR4 was confirmed with Western blots on lung nuclear extracts. We conclude that TLR4 expression in lung is sustained up to 36 hours and that TLR4 and LPS are localized in the cytoplasm and nuclei of lung cellses
dc.formatapplication/pdfes
dc.format.extent10es
dc.identifier.issn0213-3911es
dc.identifier.urihttp://hdl.handle.net/10201/22682
dc.languageenges
dc.publisherMurcia : F. Hernándezes
dc.relation.ispartofHistology and histopathologyes
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.subjectInnate immunityes
dc.subjectImmunohistochemistryes
dc.subject.otherCDU::6 - Ciencias aplicadas::61 - Medicina::616 - Patología. Medicina clínica. Oncologíaes
dc.titleToll like receptor-4 expression in lipopolysaccharide induced lung inflammationes
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
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Histology and histopathology Vol.21, nº 7 (2006)