Publication: Toll like receptor-4 expression in lipopolysaccharide induced lung inflammation
Authors
Janardhan, K. ; McIsaac, M. ; Fowlie, J. ; Shrivastav, A. ; Caldwell, S. ; Sharma, R.K. ; Singh, B.
item.page.secondaryauthor
item.page.director
Publisher
Murcia : F. Hernández
publication.page.editor
publication.page.department
DOI
item.page.type
info:eu-repo/semantics/article
Description
Abstract
Bacterial lipopolysaccharides (LPS) initiate
immune response through Toll-like receptor 4 (TLR4).
Because many a times host is confronted with secondary
bacterial challenges, it is critical to understand TLR4
expression following initial provocation. We studied
TLR4 expression in rats at various times after intratracheal
instillation of LPS. Although TLR4 mRNA was
undetectable in normal lungs, it increased at 6h and 12h
and declined at 36h post-LPS treatment. Western blots
showed TLR4 protein at all time points.
Immunohistochemistry localized TLR4 in alveolar septal
cells, bronchial epithelium, macrophages and
endothelium of large and peribronchial blood vessels.
Dual label immunoelectron microscopy showed colocalization
of TLR4 and LPS in the cytoplasm and
nucleus of various lung and inflammatory cells. Nuclear
localization of TLR4 was confirmed with Western blots
on lung nuclear extracts. We conclude that TLR4
expression in lung is sustained up to 36 hours and that
TLR4 and LPS are localized in the cytoplasm and nuclei
of lung cells
publication.page.subject
Citation
item.page.embargo
Ir a Estadísticas
Sin licencia Creative Commons.