Publication: Induction of DNA fragmentation by total-body irradiation in murine liver
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Date
1998
Authors
Terashima, M. ; Ogawal, Y. ; Toda, K. ; Hamada, N. ; Nishioka, A. ; Inomata, T. ; Yoshida, S. ; Shizuta, Y. ; Seguchi, H.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Total-body irradiation (TBI) is an accepted
modality to treat patients with disseminated tumors. The
influence of the treatment on normal tissues is evaluated
using mice by measuring the rate of the induction and
distribution of apoptosis, as well as DNA fragmentation
which occurs in the murine liver within hours of
irradiation.
Unanesthetized female C3H/He mice were exposed
to y-ray TB1 of 2, 7, and 20 gray (Gy) delivered from
6 0 ~ aot a dose rate of 114 cGy/min. Frozen sections of
livers which were excised from the animals at various
times after irradiation were stained by hematoxylin-eosin
(H-E) to count numbers of apoptotic cells, or were
examined to detect DNA fragmentation.
The percentages of apoptotic cells and length of the
period during which the maximum levels of the
percentages were exhibited showed a dose-dependent
increase in the sections stained with H-E. No positive
cells for 3'-OH ends of fragmented DNA were found in
the liver before TBI, whereas positive cells were
observed immediately after irradiation without dosedependency,
these positive cells returned to nearly basal
levels after several hours. Positive cells were observed
prior to showing apoptosis, suggesting that DNA
fragmentation occurs immediately after TB1 independent
of apoptosis. The difference in the time courses between
induction of DNA fragmentation and of apoptosis was
not observed in other organs or in the samples treated
with the detergent. These results suggested that the 3'-
OH ends newly generated by TB1 were masked by a
detergent-soluble DNA-binding molecule which might
be preferentially present in the murine liver.
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