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Histological examination on osteoblastic activities in the alveolar bone of transgenic mice with induced ablation of osteocytes

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dc.contributor.authorLi, Minqi
dc.contributor.authorHasegawa, Tomoka
dc.contributor.authorHogo, Hiromi
dc.contributor.authorTatsumi, Sawako
dc.contributor.authorLiu, Zhusheng
dc.contributor.authorGuo, Ying
dc.contributor.authorSasaki, Muneteru
dc.contributor.authorTabata, Chihiro
dc.contributor.authorYamamoto, Tsuneyuki
dc.contributor.authorIkeda, Kyoji
dc.contributor.authorAmizuka, Norio
dc.date.accessioned2018-02-16T14:54:39Z
dc.date.available2018-02-16T14:54:39Z
dc.date.issued2013
dc.description.abstractThe purpose of this study was to examine histological alterations on osteoblasts from the alveolar bone of transgenic mice with targeted ablation of osteoctyes. Eighteen weeks-old transgenic mice based on the diphtheria toxin (DT) receptor-mediated cell knockout (TRECK) system were used in these experiments. Mice were injected intraperitoneally with 50 µg/kg of DT in PBS, or only PBS as control. Two weeks after injections, mice were subjected to transcardiac perfusion with 4% paraformaldehyde in 0.1M phosphate buffer (pH 7.4), and the available alveolar bone was removed for histochemical analyses. Approximately 75% of osteocytes from alveolar bones became apoptotic after DT administration, and most osteocytic lacunae became empty. Osteoblastic numbers and alkaline phosphatase (ALP) activity were markedly reduced at the endosteum of alveolar bone after DT administration compared with the control. Osteoblastic ALP activity in the periodontal ligament region, on the other hand, hardly showed any differences between the two groups even though numbers were reduced in the experiment group. Silver impregnation showed a difference in the distribution of bone canaliculi between the portions near the endosteum and the periodontal ligament: the former appeared regularly arranged in contrast to the latter’s irregular distribution. Under transmission electron microscopy (TEM), the osteoblasts in the periodontal ligament showed direct contact with the Sharpey’s fibers. Thus, osteoblastic activity was affected by osteocyte ablation in general, but osteoblasts in contact with the periodontal ligament were less affected than endosteal osteoblasts.es
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dc.format.extent9es
dc.identifier.citationHistology and histopathology, Vol. 28, n.º 3 (2013)
dc.identifier.issn1699-5848
dc.identifier.issn0213-3911
dc.identifier.urihttp://hdl.handle.net/10201/56175
dc.languageenges
dc.publisherF. Hernández y Juan F. Madrid. Universidad de Murcia. Departamento de Biología Celular e Histologíaes
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.subjectAlveolar bonees
dc.subjectOsteocytees
dc.subject.otherCDU::5 - Ciencias puras y naturales::57 - Biología::576 - Biología celular y subcelular. Citologíaes
dc.titleHistological examination on osteoblastic activities in the alveolar bone of transgenic mice with induced ablation of osteocyteses
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
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Histology and histopathology Vol.28, nº 3 (2013)