Publication: Histological examination on osteoblastic
activities in the alveolar bone of transgenic
mice with induced ablation of osteocytes
Authors
Li, Minqi ; Hasegawa, Tomoka ; Hogo, Hiromi ; Tatsumi, Sawako ; Liu, Zhusheng ; Guo, Ying ; Sasaki, Muneteru ; Tabata, Chihiro ; Yamamoto, Tsuneyuki ; Ikeda, Kyoji ; Amizuka, Norio
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Publisher
F. Hernández y Juan F. Madrid. Universidad de Murcia. Departamento de Biología Celular e Histología
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DOI
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info:eu-repo/semantics/article
Description
Abstract
The purpose of this study was to examine
histological alterations on osteoblasts from the alveolar
bone of transgenic mice with targeted ablation of
osteoctyes. Eighteen weeks-old transgenic mice based
on the diphtheria toxin (DT) receptor-mediated cell
knockout (TRECK) system were used in these
experiments. Mice were injected intraperitoneally with
50 µg/kg of DT in PBS, or only PBS as control. Two
weeks after injections, mice were subjected to
transcardiac perfusion with 4% paraformaldehyde in
0.1M phosphate buffer (pH 7.4), and the available
alveolar bone was removed for histochemical analyses.
Approximately 75% of osteocytes from alveolar bones
became apoptotic after DT administration, and most
osteocytic lacunae became empty. Osteoblastic numbers
and alkaline phosphatase (ALP) activity were markedly
reduced at the endosteum of alveolar bone after DT
administration compared with the control. Osteoblastic
ALP activity in the periodontal ligament region, on the
other hand, hardly showed any differences between the
two groups even though numbers were reduced in the
experiment group. Silver impregnation showed a
difference in the distribution of bone canaliculi between
the portions near the endosteum and the periodontal
ligament: the former appeared regularly arranged in
contrast to the latter’s irregular distribution. Under
transmission electron microscopy (TEM), the osteoblasts
in the periodontal ligament showed direct contact with
the Sharpey’s fibers. Thus, osteoblastic activity was
affected by osteocyte ablation in general, but osteoblasts
in contact with the periodontal ligament were less
affected than endosteal osteoblasts.
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Citation
Histology and histopathology, Vol. 28, n.º 3 (2013)
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