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Model of human recurrent respiratory papilloma on chicken embryo chorioallantoic membrane for tumor angiogenesis research

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dc.contributor.authorUloza, Virgilijus
dc.contributor.authorKuzminienė, Alina
dc.contributor.authorPalubinskienė, Jolita
dc.contributor.authorBalnytė, Ingrida
dc.contributor.authorUlozienė, Ingrida
dc.contributor.authorValančiūtė, Angelija
dc.date.accessioned2022-02-24T09:53:54Z
dc.date.available2022-02-24T09:53:54Z
dc.date.issued2017
dc.description.abstractWe aimed to develop a chick embryo chorioallantoic membrane (CAM) model of recurrent respiratory papilloma (RPP) and to evaluate its morphological and morphometric characteristics, together with angiogenic features. Fresh RRP tissue samples obtained from 13 patients were implanted in 174 chick embryo CAMs. Morphological, morphometric, and angiogenic changes in the CAM and chorionic epithelium were evaluated up until 7 days after the implantation. Immunohistochemical analysis (34βE12, Ki-67, MMP-9, PCNA, and Sambucus nigra staining) was performed to detect cytokeratins and endothelial cells and to evaluate proliferative capacity of the RRP before and after implantation on the CAM. The implanted RRP tissue samples survived on CAM in 73% of cases while retaining their essential morphologic characteristics and proliferative capacity of the original tumor. Implants induced thickening of both the CAM (241-560%, p=0.001) and the chorionic epithelium (107-151%, p=0.001), while the number of blood vessels (37-85%, p=0.001) in the CAM increased. The results of the present study confirmed that chick embryo CAM is a relevant host for serving as a medium for RRP fresh tissue implantation. The CAM assay demonstrated the specific RRP tumor growth pattern after implantation and provided the first morphological and morphometric characterization of the RRP CAM model that opens new horizons in studying this disease.es
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dc.identifier.citationHistology and Histopathology, Vol.32, nº7, (2017)
dc.identifier.doiDOI: 10.14670/HH-11-831
dc.identifier.issn1699-5848
dc.identifier.issn0213-3911
dc.identifier.urihttp://hdl.handle.net/10201/117345
dc.languageenges
dc.publisherUniversidad de Murcia. Departamento de Biología Celular e Histologíaes
dc.relationSin financiación externa a la Universidades
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectRecurrent respiraory papillomaes
dc.subjectChoriallantoic membranees
dc.subjectAngiogenesises
dc.subject.otherCDU::6 - Ciencias aplicadas::61 - Medicina::616 - Patología. Medicina clínica. Oncologíaes
dc.titleModel of human recurrent respiratory papilloma on chicken embryo chorioallantoic membrane for tumor angiogenesis researches
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
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Histology and histopathology Vol.32, nº7 (2017)