Publication: Model of human recurrent respiratory papilloma on chicken embryo chorioallantoic membrane for tumor angiogenesis research
Authors
Uloza, Virgilijus ; Kuzminienė, Alina ; Palubinskienė, Jolita ; Balnytė, Ingrida ; Ulozienė, Ingrida ; Valančiūtė, Angelija
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Publisher
Universidad de Murcia. Departamento de Biología Celular e Histología
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DOI
DOI: 10.14670/HH-11-831
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info:eu-repo/semantics/article
Description
Abstract
We aimed to develop a chick embryo
chorioallantoic membrane (CAM) model of recurrent
respiratory papilloma (RPP) and to evaluate its
morphological and morphometric characteristics,
together with angiogenic features.
Fresh RRP tissue samples obtained from 13 patients
were implanted in 174 chick embryo CAMs.
Morphological, morphometric, and angiogenic changes
in the CAM and chorionic epithelium were evaluated up
until 7 days after the implantation. Immunohistochemical analysis (34βE12, Ki-67, MMP-9, PCNA, and
Sambucus nigra staining) was performed to detect
cytokeratins and endothelial cells and to evaluate
proliferative capacity of the RRP before and after
implantation on the CAM.
The implanted RRP tissue samples survived on
CAM in 73% of cases while retaining their essential
morphologic characteristics and proliferative capacity of
the original tumor. Implants induced thickening of both
the CAM (241-560%, p=0.001) and the chorionic
epithelium (107-151%, p=0.001), while the number of
blood vessels (37-85%, p=0.001) in the CAM increased.
The results of the present study confirmed that chick
embryo CAM is a relevant host for serving as a medium
for RRP fresh tissue implantation. The CAM assay
demonstrated the specific RRP tumor growth pattern
after implantation and provided the first morphological
and morphometric characterization of the RRP CAM
model that opens new horizons in studying this disease.
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Citation
Histology and Histopathology, Vol.32, nº7, (2017)
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