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The role of proteoglycans in maintaining collagen fibril morphology

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dc.contributor.authorDell`Orbo, Carloes
dc.contributor.authorDe Luca, G.es
dc.contributor.authorGioglio, L.
dc.contributor.authorQuacci, Daniela
dc.contributor.authorSoldi, C.
dc.date.accessioned2011-02-22T10:30:51Z
dc.date.available2011-02-22T10:30:51Z
dc.date.issued1995
dc.description.abstractThe aortic wall contains various heterogeneous proteoglycan populations which interact in different ways with other components of extracellular matrix. Proteoglycans (PGs) are known to provide structural support to the vessel wall as well as to influence specific physiological functions of the tissues. The aim of the present study was to investigate the effects of Chondroitinase AC (Chase), Streptococcal Hyaluronidase (Hyase) and Heparanase on human aortic wall collagen which had been treated previously with 4M GuHCl, in order to verify the effects of selective glycanolytic treatment on type 1 collagen fibril ultrastructure. Following 4M GuHCl treatment, collagen fibrils are seen to have a clearly visible period. Subsequent to GuHCl and Streptococcal Hyase treatment al1 collagen fibrils appear to be completely swollen in thin aperiodic filaments; the typical 64 nm collagen period is completely undetectable. After GuHCl and Chase treatment a small number of collagen fibrils are seen to be swollen in thin fibrils which are mainly localized at some distance from elastic fibres. Following GuHCl and HeparanaseIHeparitinase 111 treatment a considerable number of collagen fibrils appear to be swollen in thin fibrils; the majority of which are situated in the vicinity of elastic fibrils. The swelling of collagen fibrils underlines the fundamental role of proteoglycans in maintaining collagen fibril integrity and periodicity. It is as yet impossible to precisely map interactions between these proteoglycans and collagen fibres. The role of Hyaluronic acid requires further investigation, although the nature of this interaction is undoubtedly a matter of considerable interest.es
dc.formatapplication/pdfes
dc.format.extent6es
dc.identifier.issn0213-3911es
dc.identifier.urihttp://hdl.handle.net/10201/18760
dc.languageenges
dc.publisherMurcia : F. Hernándezes
dc.relation.ispartofHistology and histopathologyes
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.subjectCollagen fibrilses
dc.subjectGlvcanolvtic digestiones
dc.subject.otherCDU::6 - Ciencias aplicadas::61 - Medicina::611 - Anatomíaes
dc.titleThe role of proteoglycans in maintaining collagen fibril morphologyes
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
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Histology and histopathology Vol.10, nº 3 (1995)