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Immunohistochemical assessment of cell populations in leprosy-spectrum lesions and reactional forms

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dc.contributor.authorFachin, Luciana Raquel Vincenzi
dc.contributor.authorSoares, Cleverson Teixeira
dc.contributor.authorBelone, Andrea de Faria Fernandes
dc.contributor.authorTrombone, Ana Paula Favaro
dc.contributor.authorRosa, Patrícia Sammarco
dc.contributor.authorGuidella, Cássio Cesar
dc.contributor.authorFranco, Marcello Fabiano
dc.date.accessioned2022-02-17T11:18:00Z
dc.date.available2022-02-17T11:18:00Z
dc.date.issued2017
dc.description.abstractIn situ immunophenotyping of leprosy lesions can improve our understanding of the biology of inflammatory cells during the immune response to Mycobacterium leprae antigens. In the present study, biopsies from 10 healthy controls and 70 leprosy patients were selected, 10 for each of the following conditions: clinical tuberculoid (TT), borderline tuberculoid (BT), borderline borderline (BB), borderline lepromatous (BL), lepromatous (LL), reversal reaction (R1), and erythema nodosum leprosum (R2). Qualitative and quantitative immunohistochemical analyses were performed to detect CD3, CD4, CD8, FoxP3, CD20, CD138, CD1a, CD57, CD15, CD117, CD68, and CD163. In addition, histochemistry was employed to identify eosinophils. The amount of CD3+ and CD4+ T cells was higher in TT than in LL patients. CD8+ T cells were predominant in T lymphocyte infiltrations in the basal layer of the epidermis. The number of FoxP3+ cells was similar among different forms of the disease, but was higher in BL and LL than in R2 individuals. CD20+ lymphocytes were most abundant in TT samples, while CD138+ plasma cells displayed no detectable differences. Epithelioid macrophages from the center of TT and R1 granulomas exhibited the M1 phenotype (CD68+CD163- ), whereas those in LL granulomas showed the M2 phenotype (CD68+CD163+). There was a gradual decrease in the amount of CD1a+ cells from the TT towards the LL form of the disease. A significant increase in the number of neutrophils was observed only in R2 samples. All the cells investigated, except eosinophils, participated in the immunopathogenesis of leprosy.es
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dc.format.extent12es
dc.identifier.citationHistology and Histopathology, Vol.32, nº4, (2017)
dc.identifier.doiDOI: 10.14670/HH-11-804
dc.identifier.issn1699-5848
dc.identifier.issn0213-3911
dc.identifier.urihttp://hdl.handle.net/10201/117129
dc.languageenges
dc.publisherUniversidad de Murcia. Departamento de Biología Celular e Histologíaes
dc.relationSin financiación externa a la Universidades
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectLeprosyes
dc.subjectM. lepraees
dc.subjectPathogenesises
dc.subjectCellular infiltratees
dc.subjectImmunopathologyes
dc.subject.otherCDU::6 - Ciencias aplicadas::61 - Medicina::616 - Patología. Medicina clínica. Oncologíaes
dc.titleImmunohistochemical assessment of cell populations in leprosy-spectrum lesions and reactional formses
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
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Histology and histopathology Vol.32, nº4 (2017)