Publication:
Enhanced expression of endothelin-1 and endothelinconverting enzyme-1 in acute hypoxic rat aorta

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dc.contributor.authorDoi, Y.es
dc.contributor.authorKudo, H.es
dc.contributor.authorNishino, T.
dc.contributor.authorYamamoto, O.
dc.contributor.authorNagata, T.
dc.contributor.authorNara, S.
dc.contributor.authorMorita, M.
dc.contributor.authorFujimoto, S.
dc.date.accessioned2011-05-11T10:57:47Z
dc.date.available2011-05-11T10:57:47Z
dc.date.issued2002
dc.description.abstractDeeply anesthetized male Wistar rats were perfused by Hanks’ balanced salt solution bubbled with either 95%air and 5%CO2 (normoxic group) or 95%N2 and 5%CO2 (hypoxic group) from the thoracic aorta for 30 min, and the isolated abdominal aortae from both groups were used for electron microscopy, immunocytochemistry of endothelin (ET)-1 and ETconverting enzyme (ECE)-1, and in situ hybridization of p r e p r o E T-1 mRNA. A remarkable increase in the number of Weibel-Palade (WP) bodies, storage sites of E T-1 and ECE-1, occurred in the hypoxic group when compared to the normoxic group. Immunoreactivities for E T-1 and ECE-1, and signals for preproET-1 mRNA were seen along the endothelia of both groups, but the intensities were significantly elevated in the hypoxic group. The increase in the number of ECE-1 immunoreactive gold particles was noticed especially in WP bodies in the hypoxic group. These findings indicate the enhancement of preproET-1 synthesis in the aortic endothelial cells as well as the acceleration of ET- 1 processing in increased WP bodies in such cells in an experimentally hypoxic condition of the rat aortae.es
dc.formatapplication/pdfes
dc.format.extent9es
dc.identifier.issn0213-3911es
dc.identifier.urihttp://hdl.handle.net/10201/20764
dc.languageenges
dc.publisherMurcia : F. Hernándezes
dc.relation.ispartofHistology and histopathologyes
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.subjectHypoxiaes
dc.subjectRat aortaes
dc.subject.otherCDU::6 - Ciencias aplicadas::61 - Medicinaes
dc.titleEnhanced expression of endothelin-1 and endothelinconverting enzyme-1 in acute hypoxic rat aortaes
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
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Histology and histopathology Vol.17, nº 1 (2002)