Publication: Enhanced expression of endothelin-1 and endothelinconverting
enzyme-1 in acute hypoxic rat aorta
Authors
Doi, Y. ; Kudo, H. ; Nishino, T. ; Yamamoto, O. ; Nagata, T. ; Nara, S. ; Morita, M. ; Fujimoto, S.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Deeply anesthetized male Wistar rats were
perfused by Hanks’ balanced salt solution bubbled with
either 95%air and 5%CO2 (normoxic group) or 95%N2
and 5%CO2 (hypoxic group) from the thoracic aorta for
30 min, and the isolated abdominal aortae from both
groups were used for electron microscopy,
immunocytochemistry of endothelin (ET)-1 and ETconverting
enzyme (ECE)-1, and in situ hybridization of
p r e p r o E T-1 mRNA. A remarkable increase in the
number of Weibel-Palade (WP) bodies, storage sites of
E T-1 and ECE-1, occurred in the hypoxic group when
compared to the normoxic group. Immunoreactivities for
E T-1 and ECE-1, and signals for preproET-1 mRNA
were seen along the endothelia of both groups, but the
intensities were significantly elevated in the hypoxic
group. The increase in the number of ECE-1
immunoreactive gold particles was noticed especially in
WP bodies in the hypoxic group. These findings indicate
the enhancement of preproET-1 synthesis in the aortic
endothelial cells as well as the acceleration of ET- 1
processing in increased WP bodies in such cells in an
experimentally hypoxic condition of the rat aortae.
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