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Hydroxyurea(HU)-induced apoptosis in the mouse fetal tissues

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dc.contributor.authorWoo, G.H.es
dc.contributor.authorKatayama, K.es
dc.contributor.authorJung, J.Y.
dc.contributor.authorUetsuka, K.
dc.contributor.authorBak, E.J.
dc.contributor.authorNakayama, Hiroyuki
dc.date.accessioned2011-06-08T09:03:02Z
dc.date.available2011-06-08T09:03:02Z
dc.date.issued2003
dc.description.abstractHydroxyurea (HU), a ribonucleotide reductase inhibitor, induces morphological anomalies in the central nervous system (CNS), craniofacial tissues and limb buds in animals, and neonatal respiratory distress in humans. In the present study, pregnant mice were treated with 400 mg/kg of HU at day 13 of gestation, and their fetuses were examined from 1 to 48 hours after treatment (HAT) to find a clue to clarify the mechanisms of HU-induced fetotoxicity and teratogenecity. At 6 and 12 HAT, a moderate to marked increase in the number of pyknotic cells was detected in the CNS and lung. A mild increase in the number of pyknotic cells was also found in the craniofacial mesenchymal tissues, limb buds and so on. These pyknotic cells had nuclei positively stained by the TUNEL method, which is widely used for the detection of apoptotic nuclei, and they also showed electron microscopic characteristics identical to those of apoptotic cells. The present results suggest that the HUinduced fetotoxicity is characterized by excess apoptotic cell death in the fetal tissues, and that such excess cell death in the fetal CNS, lung, craniofacial tissue and limb bud may have a certain relation to the later occurrence of morphological or functional anomalies reported in these tissues following HU-administration.es
dc.formatapplication/pdfes
dc.format.extent6es
dc.identifier.issn0213-3911es
dc.identifier.urihttp://hdl.handle.net/10201/21434
dc.languageenges
dc.publisherMurcia : F. Hernándezes
dc.relation.ispartofHistology and histopathologyes
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.subjectApoptosises
dc.subjectFetuses
dc.subject.otherCDU::6 - Ciencias aplicadas::61 - Medicinaes
dc.titleHydroxyurea(HU)-induced apoptosis in the mouse fetal tissueses
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
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Histology and histopathology Vol.18, nº 2 (2003)