Publication: Hydroxyurea(HU)-induced
apoptosis in the mouse fetal tissues
Authors
Woo, G.H. ; Katayama, K. ; Jung, J.Y. ; Uetsuka, K. ; Bak, E.J. ; Nakayama, Hiroyuki
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Hydroxyurea (HU), a ribonucleotide
reductase inhibitor, induces morphological anomalies in
the central nervous system (CNS), craniofacial tissues
and limb buds in animals, and neonatal respiratory
distress in humans. In the present study, pregnant mice
were treated with 400 mg/kg of HU at day 13 of
gestation, and their fetuses were examined from 1 to 48
hours after treatment (HAT) to find a clue to clarify the
mechanisms of HU-induced fetotoxicity and
teratogenecity. At 6 and 12 HAT, a moderate to marked
increase in the number of pyknotic cells was detected in
the CNS and lung. A mild increase in the number of
pyknotic cells was also found in the craniofacial
mesenchymal tissues, limb buds and so on. These
pyknotic cells had nuclei positively stained by the
TUNEL method, which is widely used for the detection
of apoptotic nuclei, and they also showed electron
microscopic characteristics identical to those of
apoptotic cells. The present results suggest that the HUinduced
fetotoxicity is characterized by excess apoptotic
cell death in the fetal tissues, and that such excess cell
death in the fetal CNS, lung, craniofacial tissue and limb
bud may have a certain relation to the later occurrence of
morphological or functional anomalies reported in these
tissues following HU-administration.
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