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Topical fluoride varnishes promote several biological responses on human gingival cells

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dc.contributor.authorLópez García, Sergio
dc.contributor.authorPecci Lloret, María Pilar
dc.contributor.authorPecci Lloret, Miguel Ramón
dc.contributor.authorGuerrero Gironés, Julia
dc.contributor.authorRodríguez Lozano, Francisco Javier
dc.contributor.authorGarcía Bernal, David
dc.contributor.departmentDermatología, Estomatología, Radiología y Medicina Física
dc.date.accessioned2025-01-28T17:12:08Z
dc.date.available2025-01-28T17:12:08Z
dc.date.issued2021-04-18
dc.description© 2021, Elsevier. This document is the Published version of a Published Work that appeared in final form in Annals of Anatomy. To access the final edited and published work see https://doi.org/10.1016/j.aanat.2021.151829
dc.description.abstractObjective: To compare the in vitro cytotoxicity of four commercial topical fluoride varnishes widely used in daily dental practice for the prevention of caries on human fibroblasts: Cervitec F, Fixofluor, Fluor Protector S and Duraphat. Material and methods: Human gingival fibroblasts (hGF) were exposed to different concentrations of fluoride varnishes extracts. Biological assays, including MTT and IC50 value determination, annexin-V/7- AAD staining, cell migration and F-actin staining with phalloidin were carried out. Statistical analyses were performed using one-way ANOVA and Tukey’s post hoc test. Results: At 4% concentration, all of the fluoride varnishes extracts affected fibroblasts metabolic activity, exhibiting ahighdegree of cytotoxicity at allmeasured time points.At 0.1%and 1%, Duraphat and Fixofluor or Fluor Protector S and Cervitec F exerted the lowest or highest cytotoxic effects, respectively. Similar effects were evidenced when induction of apoptosis/necrosis and cell migration assays were analyzed. Immunocytochemical assays revealed a similarnumber offibroblasts, without changes inthemorphology and F-actin content at 0.1% concentration of alltested materials, while at 1% concentration, Fluor Protector S and Cervitec F showed few cells with aberrant morphology or non-adhered cells, respectively. Conclusions: Different commercial topical fluoride varnishes with the same therapeutic indication may exhibit different biological effects and cytotoxicity on fibroblasts.
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dc.identifier.citationAnnals of Anatomy, 2021, Vol. 237 151723
dc.identifier.doihttps://doi.org/10.1016/j.aanat.2021.151829
dc.identifier.issnPrint: 0940-9602
dc.identifier.issnElectronic: 1618-0402
dc.identifier.urihttp://hdl.handle.net/10201/149492
dc.languageenges
dc.publisherElsevier
dc.relationLa financiación para el estudio procedía del proyecto de la Red TerCel financiado por el Instituto Carlos III, siendo un proyecto competitivo nacional denominado RD16/0011/001 RED DE TERAPIA CELULAR.es
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S0940960221000492?via%3Dihub
dc.rights.accessRightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectFluoride varnisheses
dc.subjectCytotoxicity
dc.subjectGingival cells
dc.subjectBiological effects
dc.titleTopical fluoride varnishes promote several biological responses on human gingival cellses
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
relation.isAuthorOfPublication2372b160-a380-45dd-835a-6f377fbeaf97
relation.isAuthorOfPublication.latestForDiscovery2372b160-a380-45dd-835a-6f377fbeaf97
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