Publication: Topical fluoride varnishes promote several biological responses on human gingival cells
Authors
López García, Sergio ; Pecci Lloret, María Pilar ; Pecci Lloret, Miguel Ramón ; Guerrero Gironés, Julia ; Rodríguez Lozano, Francisco Javier ; García Bernal, David
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Publisher
Elsevier
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DOI
https://doi.org/10.1016/j.aanat.2021.151829
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info:eu-repo/semantics/article
Description
© 2021, Elsevier. This document is the Published version of a Published Work that appeared in final form in Annals of Anatomy. To access the final edited and published work see https://doi.org/10.1016/j.aanat.2021.151829
Abstract
Objective: To compare the in vitro cytotoxicity of four commercial topical fluoride varnishes widely used in daily dental practice for the prevention of caries on human fibroblasts: Cervitec F, Fixofluor, Fluor Protector S and Duraphat. Material and methods: Human gingival fibroblasts (hGF) were exposed to different concentrations of fluoride varnishes extracts. Biological assays, including MTT and IC50 value determination, annexin-V/7-
AAD staining, cell migration and F-actin staining with phalloidin were carried out. Statistical analyses were performed using one-way ANOVA and Tukey’s post hoc test. Results: At 4% concentration, all of the fluoride varnishes extracts affected fibroblasts metabolic activity, exhibiting ahighdegree of cytotoxicity at allmeasured time points.At 0.1%and 1%, Duraphat and Fixofluor or Fluor Protector S and Cervitec F exerted the lowest or highest cytotoxic effects, respectively. Similar effects were evidenced when induction of apoptosis/necrosis and cell migration assays were analyzed.
Immunocytochemical assays revealed a similarnumber offibroblasts, without changes inthemorphology and F-actin content at 0.1% concentration of alltested materials, while at 1% concentration, Fluor Protector S and Cervitec F showed few cells with aberrant morphology or non-adhered cells, respectively. Conclusions: Different commercial topical fluoride varnishes with the same therapeutic indication may exhibit different biological effects and cytotoxicity on fibroblasts.
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Citation
Annals of Anatomy, 2021, Vol. 237 151723
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