Publication:
Quantitative detection of anionic sites in rat femoral velscartilage using cationic colloidal gold at low pH le

Simple item page
dc.contributor.authorUeda, H.es
dc.contributor.authorKato, Y.es
dc.contributor.authorOhno, S.
dc.date.accessioned2011-02-22T10:59:41Z
dc.date.available2011-02-22T10:59:41Z
dc.date.issued1998
dc.description.abstractQuantitative analyses of anionic sites in the rat cartilage were performed by using post-embedding LR-Gold method with cationic colloidal gold (CCG) at different pH levels. Alteration of the pH levels affected the CCG staining patterns. The CCG particles were more observed at low pH levels, 1.0 and 2.5, than those at high pH 7.4. So, we quantitatively examined the superficial, intermediate and deep layers at pH 1.0. Morphometric analyses showed that CCG particles were increased from the superficial layer to the deep layer in both pericellular matrix (PM) and interterritorial matrix (IM). Moreover, they were more observed in PM than in IM. After chondroitinase ABC digestion, the CCG labeling was reduced in all matrices of the superficial and intermediate layers, though many CCG particles were still observed in PM of the deep layer. After hyaluronidase digestion, the CCG labeling was markedly reduced in the PM of the deep layer. These findings suggest that anionic sites in the cartilage matrix are detected by using the CCG only at low pH levels. In addition, the PM in the deep layer may consist of different components from those of the other two layers.es
dc.formatapplication/pdfes
dc.format.extent9es
dc.identifier.issn0213-3911es
dc.identifier.urihttp://hdl.handle.net/10201/19114
dc.languageenges
dc.publisherMurcia : F. Hernándezes
dc.relation.ispartofHistology and histopathologyes
dc.rightsinfo:eu-repo/semantics/openAccesses
dc.subjectHyaline cartilagees
dc.subjectProteoglycanes
dc.subject.otherCDU::6 - Ciencias aplicadas::61 - Medicinaes
dc.titleQuantitative detection of anionic sites in rat femoral velscartilage using cationic colloidal gold at low pH lees
dc.typeinfo:eu-repo/semantics/articlees
dspace.entity.typePublicationes
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Quantitative detection of anionic sites in rat femoral velscartilage using cationic colloidal gold at low pH le.pdf
Size:
5.04 MB
Format:
Adobe Portable Document Format
Description:
Download
Collections
Histology and histopathology Vol.13, nº 4 (1998)