Publication: Quantitative detection of anionic sites in rat femoral velscartilage using cationic colloidal gold at low pH le
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Date
1998
Authors
Ueda, H. ; Kato, Y. ; Ohno, S.
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Publisher
Murcia : F. Hernández
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DOI
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info:eu-repo/semantics/article
Description
Abstract
Quantitative analyses of anionic sites in the
rat cartilage were performed by using post-embedding
LR-Gold method with cationic colloidal gold (CCG) at
different pH levels. Alteration of the pH levels affected
the CCG staining patterns. The CCG particles were more
observed at low pH levels, 1.0 and 2.5, than those at
high pH 7.4. So, we quantitatively examined the
superficial, intermediate and deep layers at pH 1.0.
Morphometric analyses showed that CCG particles were
increased from the superficial layer to the deep layer in
both pericellular matrix (PM) and interterritorial matrix
(IM). Moreover, they were more observed in PM than in
IM. After chondroitinase ABC digestion, the CCG
labeling was reduced in all matrices of the superficial
and intermediate layers, though many CCG particles
were still observed in PM of the deep layer. After
hyaluronidase digestion, the CCG labeling was markedly
reduced in the PM of the deep layer. These findings
suggest that anionic sites in the cartilage matrix are
detected by using the CCG only at low pH levels. In
addition, the PM in the deep layer may consist of
different components from those of the other two
layers.
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